Overexpression of HOXB7 is associated with a poor prognosis in patients with gastric cancer.
Overexpression of HOXB7 is associated with a poor prognosis in patients with gastric cancer.
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DOI：
10.3892/ol.2015.3630
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发表时间：
2015-11
期刊：
影响因子：
2.9
通讯作者：
Wei‐wei Tu;Xingwu Zhu;Yang Han;Yugang Wen;G. Qiu;Chongzhi Zhou
中科院分区：
文献类型：
--
作者：
Wei‐wei Tu;Xingwu Zhu;Yang Han;Yugang Wen;G. Qiu;Chongzhi Zhou
此前的研究表明，同源盒基因HOXB7在某些癌症中过度表达，从而促进肿瘤发生。然而，人们对 HOXB7 基因与胃癌之间的关系知之甚少。本研究的目的是探讨HOXB7表达水平与胃癌之间的关系。采用逆转录定量聚合酶链反应和蛋白质印迹分析分别检测同源盒B7（HOXB7）RNA和蛋白的表达。此外，通过免疫组化分析HOXB7的表达与胃癌临床病理特征的关系。采用Kaplan-Meier法计算生存率，采用COX比例风险模型进行单变量和多变量分析。与相应的正常粘膜相比，癌组织中HOXB7 RNA和蛋白的表达水平显着升高。 HOXB7表达增加与肿瘤大小（P=0.01）、T分期（P<0.001）和国际癌症控制联盟晚期分期（P=0.003）显着相关。此外，与不表达HOXB7的肿瘤患者相比，HOXB7表达阳性的患者的总生存率和无病生存率明显较低。此外，单变量和多变量分析表明 HOXB7 是胃癌患者 OS 和 DFS 的重要独立预后因素。目前的数据表明HOXB7基因可能在胃癌发生过程中发挥重要作用，也表明HOXB7可能是胃癌患者预后的重要决定因素。
Previous studies have indicated that the homeobox gene HOXB7 is overexpressed in certain cancers, which promotes tumorigenesis. However, less is known about the association between the HOXB7 gene and gastric cancer. The purpose of the present study was to investigate the association between the expression level of HOXB7 and gastric cancer. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect the expression of the homeobox B7 (HOXB7) RNA and protein, respectively. In addition, the association between the expression of HOXB7 and the clinicopathological characteristics of gastric cancer was analyzed by immunohistochemistry. The Kaplan-Meier method was used to calculate the survival rates, and the COX proportional hazards model was used to investigate univariate and multivariate analyses. The expression level of HOXB7 RNA and protein was significantly elevated in cancerous tissues compared with the corresponding normal mucosa. Increased expression of HOXB7 was significantly associated with tumor size (P=0.01), T stage (P<0.001) and advanced Union for International Cancer Control stage (P=0.003). In addition, patients with positive HOXB7 expression possessed an evident lower overall survival and disease-free survival rate compared with patients with tumors that did not express HOXB7. Furthermore, univariate and multivariate analyses indicated that HOXB7 served as a significant independent prognostic factor for OS and DFS in patients with gastric cancer. The present data indicate that the HOXB7 gene may play an important role in the process of gastric tumorigenesis, and also indicate that HOXB7 may be an important determinant of patient prognosis in gastric cancer.