Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in Arabidopsis thaliana
Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in Arabidopsis thaliana
复制标题
拟南芥冷冻和冷冻后恢复过程中质体外和质体脂质的差异降解
DOI：
10.1074/jbc.m706692200
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发表时间：
2008-01-04
影响因子：
4.8
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Li, Weiqi;Wang, Ruiping;Wang, Xuemin
膜脂组成的变化对植物适应低温环境和低温后的存活起着重要作用。植物对低温和冰冻的反应包括三个不同的阶段：低温驯化、冰冻和冰冻后的恢复。对低温驯化和冷冻过程中脂质变化的研究已经取得了相当大的进展，但对冷冻后恢复过程中脂质的变化知之甚少。我们以前发现磷脂酶D（PLD）参与脂质水解和拟南芥抗冻性。本研究旨在确定冷冻后恢复期间脂质种类如何变化，并确定两种PLD（PLD α 1和PLD δ）对冷冻后恢复期间脂质变化的影响。在冷冻后恢复期间，质体脂质、单半乳糖基二酰基甘油和质体磷脂酰甘油的水解是最显著的变化。相反，在冷冻过程中，发生质外磷脂、磷脂酰胆碱和磷脂酰乙醇胺的水解。抑制PLD α 1减少了冷冻和冷冻后阶段的磷脂水解和磷脂酸产生，而消融PLD δ增加了冷冻后恢复期间的脂质水解和磷脂酸产生。因此，在冷冻和冷冻后恢复过程中，脂质水解发生明显不同的变化。PLD α 1的存在与冷冻和冷冻后阶段的磷脂水解相关，而PLD δ的存在与冷冻后恢复期间的脂质水解减少相关。这些数据表明，PLD α 1的负作用和PLD δ在抗冻性中的积极作用。
Changes in membrane lipid composition play important roles in plant adaptation to and survival after freezing. Plant response to cold and freezing involves three distinct phases: cold acclimation, freezing, and post-freezing recovery. Considerable progress has been made toward understanding lipid changes during cold acclimation and freezing, but little is known about lipid alteration during post-freezing recovery. We previously showed that phospholipase D (PLD) is involved in lipid hydrolysis and Arabidopsis thaliana freezing tolerance. This study was undertaken to determine how lipid species change during post-freezing recovery and to determine the effect of two PLDs, PLD alpha 1 and PLD delta, on lipid changes during post-freezing recovery. During post-freezing recovery, hydrolysis of plastidic lipids, monogalactosyldiacylglycerol and plastidic phosphatidylglycerol, is the most prominent change. In contrast, during freezing, hydrolysis of extraplastidic phospholipids, phosphatidylcholine and phosphatidylethanolamine, occurs. Suppression of PLD alpha 1 decreased phospholipid hydrolysis and phosphatidic acid production in both the freezing and post-freezing phases, whereas ablation of PLD delta increased lipid hydrolysis and phosphatidic acid production during post-freezing recovery. Thus, distinctly different changes in lipid hydrolysis occur in freezing and post-freezing recovery. The presence of PLD alpha 1 correlates with phospholipid hydrolysis in both freezing and post-freezing phases, whereas the presence of PLD delta correlates with reduced lipid hydrolysis during post-freezing recovery. These data suggest a negative role for PLD alpha 1 and a positive role for PLD delta in freezing tolerance.