T-Cell Responses in Children to Internal Influenza Antigens, 1 Year After Immunization With Pandemic H1N1 Influenza Vaccine, and Response to Revaccination With Seasonal Trivalent-inactivated Influenza Vaccine
T-Cell Responses in Children to Internal Influenza Antigens, 1 Year After Immunization With Pandemic H1N1 Influenza Vaccine, and Response to Revaccination With Seasonal Trivalent-inactivated Influenza Vaccine
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DOI：
10.1097/inf.0b013e318255e443
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发表时间：
2012-06-01
影响因子：
3.6
通讯作者：
Gilbert, Sarah C.
中科院分区：
文献类型：
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作者：
Lambe, Teresa;Spencer, Alexandra J.;Gilbert, Sarah C.
背景：在季节性流感流行期间，5%-15%的人口患有一种具有不平凡的死亡率、发病率和经济负担的疾病。灭活流感疫苗通常用于预防流感感染，主要是通过诱导体液免疫。此外，三价灭活流感疫苗此前已被证明能增强一小部分成年人的流感特异性T细胞反应。我们调查了甲型H1N1流感大流行疫苗接种一年后儿童的流感特异性T细胞应答，以及三价灭活流感疫苗增强T细胞应答的能力。方法：分离曾接种甲流疫苗、2010-2011年三价流感疫苗接种前后儿童的外周血单个核细胞(PBMC)。用干扰素-γ酶联免疫吸附斑点试验分析样本对内部流感抗原(核蛋白、基质蛋白1和非结构蛋白1)的反应性。结果：干扰素-伽马酶联免疫吸附斑点试验检测结果表明，提前12个月接种AS03(B)佐剂裂解病毒大流行疫苗的儿童对核蛋白、基质蛋白1和非结构蛋白1的基础体外T细胞应答显著高于未接种全病毒疫苗的儿童。在2010/2011年季节性TIV疫苗接种后21天，无论年龄或以前的大流行疫苗接种制度，都观察到了这些反应的增强，尽管初始应答率最低的群体的增强作用更大。结论：我们在这里显示，以前接种过2009年大流行H1N1疫苗的儿童在接种后1年有可测量的T细胞反应。这些反应的大小取决于疫苗的年龄和使用的大流行性H1N1疫苗的类型。在2010/2011年度季节性TIV疫苗接种后，这些T细胞的反应经历了微小但显著的增强。
Background: During seasonal influenza epidemics, 5-15% of the population are affected with an illness having a nontrivial mortality, morbidity and economic burden. Inactivated influenza vaccines are routinely used to prevent influenza infection, primarily by inducing humoral immunity. In addition, trivalent-inactivated influenza vaccines have previously been shown to boost influenza-specific T-cell responses in a small percentage of adults. We investigate here the influenza-specific T-cell response, in children, 1 year after pandemic H1N1 vaccination and the ability to boost the T-cell response with trivalent-inactivated influenza immunization.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from children previously vaccinated with pandemic H1N1 vaccine, pre- and postseasonal 2010-2011 trivalent influenza vaccine (TIV) vaccination. Samples were analyzed by interferon-gamma enzyme-linked immunosorbent spot for reactogenicity toward internal influenza antigens (nucleoprotein, matrix protein 1 and nonstructural protein 1).Results: Basal ex vivo T-cell responses to nucleoprotein, matrix protein 1 and nonstructural protein 1 measured by interferon-gamma enzyme-linked immunosorbent spot assay were significantly higher in those children who had previously received an AS03(B)-adjuvanted split virion pandemic vaccine 12 months earlier rather than a nonadjuvanted whole virion vaccine. Boosting of these responses, 21 days after 2010/2011 seasonal TIV vaccination was observed regardless of age or prior pandemic vaccination regime, although boosting was greater in those groups with the lowest initial response.Conclusions: We show here that children previously vaccinated with the 2009 pandemic H1N1 vaccine have measurable T-cell responses 1 year after vaccination. The magnitudes of these responses are dependent on both age of vaccine and type of pandemic H1N1 vaccine used. After 2010/2011 seasonal TIV vaccination, these T-cell responses undergo a small but significant boost.