Expression of CdDHN4, a Novel YSK(2)-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway.
Expression of CdDHN4, a Novel YSK(2)-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway.
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CdDHN4（一种来自狗牙根的新型 YSK2 型脱水蛋白基因）的表达，通过 ABA 依赖性信号途径响应干旱胁迫
DOI：
10.3389/fpls.2017.00748
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhou P
中科院分区：
文献类型：
--
作者：
Lv A;Fan N;Xie J;Yuan S;An Y;Zhou P
脱氢酶可提高植物对多种非生物胁迫的抗性。本研究以狗牙根(Tifway)和C299(干旱敏感)为材料，研究了不同胁迫和脱落酸(ABA)处理下狗牙根脱水蛋白基因CDDHN4的表达谱。高温、低温、干旱、盐分和ABA均能上调该基因的表达。在干旱条件下，Tifway的CDDHN4对ABA的敏感性和表达均高于C299。用基因组步行法从Tifway克隆了一段1239bp的CDDHN4基因上游部分序列--CDDHN4-P。生物信息学分析表明，该序列具有典型的植物启动子特征，含有许多典型的顺式元件，包括转录起始点、TATA盒、AbRE、MBS、MYC、LTRE、TATC盒和GT1基序。在烟草叶片中的瞬时表达表明，CDDHN4-P启动子可以被ABA、干旱和寒冷所激活。这些结果表明，CdDHN4受ABA依赖的信号通路调控，其对ABA的高度敏感性可能是提高狗牙根抗旱性的重要机制。
Dehydrin improves plant resistance to many abiotic stresses. In this study, the expression profiles of a dehydrin gene, CdDHN4, were estimated under various stresses and abscisic acid (ABA) treatments in two bermudagrasses (Cynodon dactylon L.): Tifway (drought-tolerant) and C299 (drought-sensitive). The expression of CdDHN4 was up-regulated by high temperatures, low temperatures, drought, salt and ABA. The sensitivity of CdDHN4 to ABA and the expression of CdDHN4 under drought conditions were higher in Tifway than in C299. A 1239-bp fragment, CdDHN4-P, the partial upstream sequence of the CdDHN4 gene, was cloned by genomic walking from Tifway. Bioinformatic analysis showed that the CdDHN4-P sequence possessed features typical of a plant promoter and contained many typical cis elements, including a transcription initiation site, a TATA-box, an ABRE, an MBS, a MYC, an LTRE, a TATC-box and a GT1-motif. Transient expression in tobacco leaves demonstrated that the promoter CdDHN4-P can be activated by ABA, drought and cold. These results indicate that CdDHN4 is regulated by an ABA-dependent signal pathway and that the high sensitivity of CdDHN4 to ABA might be an important mechanism enhancing the drought tolerance of bermudagrass.