Addition of sphingosine-1-phosphate to human oocyte culture medium decreases embryo fragmentation
Addition of sphingosine-1-phosphate to human oocyte culture medium decreases embryo fragmentation
复制标题
DOI：
10.1016/j.rbmo.2009.11.020
复制
发表时间：
2010-03-01
影响因子：
4
通讯作者：
Awwad, Johnny
中科院分区：
文献类型：
--
作者：
Hannoun, Antoine;Ghaziri, Ghina;Awwad, Johnny
细胞凋亡与植入前哺乳动物胚胎的碎裂有关，但这种关联的程度仍有争议。本研究的目的是评估鞘氨醇-1-磷酸（S1 P），一种已知的抗凋亡物质，当添加到其培养微环境中时，降低人类植入前胚胎的碎片率的能力。使用胞浆内单精子注射使成熟的人卵母细胞授精，孵育3天，并由同一胚胎学家评价胚胎质量和碎片。在研究组中的卵母细胞进行操作和培养在培养基中添加S1 P至20 μ mol/l。浓度.共有46名患者为研究组捐献了177个成熟卵母细胞，为对照组捐献了546个卵母细胞。补充S1 P组的受精率明显较低（52.4%比67.3%; P = 0.002），同组中破碎率低于15%的I级胚胎比例明显较高（79.5%比53.9%; P < 0.0001）。在人类植入前胚胎的培养基中添加鞘氨醇-1-磷酸与显著较低的碎片率相关，因此胚胎质量更好。这些发现对生殖结果的临床意义仍然高度推测性，等待进一步的研究将胚胎质量的改善转化为更好的妊娠率。(C)2009年，生殖保健有限公司出版的爱思唯尔有限公司Au版权所有。
Apoptosis is implicated in the fragmentation of preimplantation mammalian embryos, yet the extent of this association remains controversial. The aim of this study was to assess the ability of sphingosine-1-phosphate (S1P), a known anti-apoptotic substance, to reduce the fragmentation rate of human preimplantation embryos when added to their culture microenvironment. Mature human oocytes were inseminated using intracytoplasmic sperm injection, incubated for 3 days and evaluated for embryo quality and fragmentation by the same embryologist. Oocytes in the study group were manipulated and cultured in culture medium supplemented with S1P to a 20 mu mol/l. concentration. A total of 46 patients donated 177 mature oocytes for the study group and 546 oocytes for the control group. The fertilization rate was significantly tower in the S1P-supplemented group (52.4% versus 67.3%; P = 0.002) and the proportion of grade I embryos with less than 15% fragmentation was significantly higher in the same group (79.5% versus 53.9%; P < 0.0001). Sphingosine-1-phosphate added to the culture medium of human preimplantation embryos is associated with a significantly lower fragmentation rate and hence better quality embryos. The clinical significance of these findings on reproductive outcome remains highly speculative awaiting further studies to translate this improvement in embryo quality into better pregnancy rates. (C) 2009, Reproductive Healthcare Ltd. Published by Elsevier Ltd. AU rights reserved.