Transinfection reveals the crucial importance of Wolbachia genotypes in determining the type of reproductive alteration in the host
Transinfection reveals the crucial importance of Wolbachia genotypes in determining the type of reproductive alteration in the host
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DOI：
10.1017/s0016672305007573
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发表时间：
2005-06-01
影响因子：
1.5
通讯作者：
Hoshizaki, S
中科院分区：
文献类型：
--
作者：
Sakamoto, H;Ishikawa, Y;Hoshizaki, S
沃尔巴克氏体是节肢动物体内的一组内共生细菌，以各种方式改变其宿主的繁殖。一个沃尔巴克氏体菌株（wSca）自然感染红小豆螟蛾Ostrinia scapulalis诱导雄性杀死，而另一个菌株（wKue）感染地中海面粉蛾Ephestia kuehniella诱导细胞质不相容性（CI）在居民主机。Wolbachia的转感染可以是一个强大的工具，阐明Wolbachia和宿主在决定生殖改变类型中的相对重要性。近年来，在E. kuehniella感染wSca。在本研究中，我们将wKue转移到O。肩胛肌的胚胎显微注射。在手术感染wKue、CI的肩胛骨，但未发生雄性死亡。因此，除了wSca之外，wKue还显示在外源宿主中诱导与其天然宿主中相同类型的改变。这些结果表明，沃尔巴克氏体基因型在决定生殖改变的类型中起着至关重要的作用。然而，本研究也揭示了宿主因素的参与。第一，测定了ivKue在O. scapulalis比E. kuehniella，表明宿主因素可影响CI水平。第二，wKue在O. scapulalis普遍较低，表明宿主影响Wolbachia传播的动态。
Wolbachia, a group of endosymbiotic bacteria in arthropods, alter the reproduction of their hosts in various ways. A Wolbachia strain (wSca) naturally infecting the adzuki bean borer moth Ostrinia scapulalis induces male killing, while another strain (wKue) infecting the Mediterranean flour moth Ephestia kuehniella induces cytoplasmic incompatibility (CI) in the resident host. Transinfection of Wolbachia can be a powerful tool to elucidate the relative importance of Wolbachia and the host in determining the type of reproductive alterations. Recently, male killing was shown to occur in E. kuehniella transinfected with wSca. In the present study, we transferred wKue to O. scapulalis by embryonic microinjection. In the O. scapulalis transinfected with wKue, CI, but not male killing occurred. Thus, in addition to wSca, wKue was shown to induce the same type of alteration in a foreign host as in its natural host. These results demonstrate the crucial role of the Wolbachia genotype in determining the type of reproductive alteration. However, the present study also revealed the involvement of host factors. First, the degree of incompatibility induced by ivKue in O. scapulalis was stronger than that in E. kuehniella, indicating that host factors can affect the level of CI. Second, the vertical transmission rate of wKue in O. scapulalis was generally low, suggesting that the host affects the dynamics of Wolbachia transmission.