Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection
Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection
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DOI：
10.1016/j.jchromb.2005.08.008
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发表时间：
2005-11-05
影响因子：
3
通讯作者：
Kiani, A
中科院分区：
文献类型：
--
作者：
Bahrami, G;Mohammadi, B;Kiani, A
建立了一种快速灵敏的高效液相色谱法测定人血清中阿托伐他汀的方法。内标双氯芬酸钠和液液萃取后，采用C18分析柱进行色谱分离，流动相为磷酸钠缓冲液（0.05 M, pH 4.0）和甲醇（33:67,v/v）。采用247nm紫外吸光度法检测阿托伐他汀和内标。本品和内标的平均加样回收率分别为95%和80%。检测下限和定量下限分别为1和4 ng/ml，在4 ~ 256 ng/ml的人血清中阿托伐他汀浓度范围内，标定曲线呈线性关系。研究了分析性能，并将该方法应用于12名健康志愿者的两种不同阿托伐他汀制剂的随机交叉生物等效性研究。(c) 2005 Elsevier B.V.版权所有
A rapid and sensitive high-performance liquid chromatographic method was validated and described for determination of atorvastatin in human serum. Following liquid-liquid extraction of the drug and an internal standard (sodium diclofenac), chromatographic separation was accomplished using C18 analytical column with a mobile phase consisting of sodium phosphate buffer (0.05 M, pH 4.0) and methanol (33:67, v/v). Atorvastatin and the internal standard were detected by ultraviolet absorbance at 247 nm. The average recoveries of the drug and internal standard were 95 and 80%, respectively. The lower limits of detection and quantification were I and 4 ng/ml, respectively, and the calibration curves were linear over a concentration range of 4-256 ng/ml of atorvastatin in human serum. The analysis performance was studied and the method was applied in a randomized cross-over bioequivalence study of two different atorvastatin preparations in 12 healthy volunteers. (c) 2005 Elsevier B.V. All rights reserved.