Effects of glutamine on adhesion molecule expression and leukocyte transmigration in endothelial cells exposed to arsenic
Effects of glutamine on adhesion molecule expression and leukocyte transmigration in endothelial cells exposed to arsenic
复制标题
DOI：
10.1016/j.jnutbio.2005.04.007
复制
发表时间：
2005-11-01
影响因子：
5.6
通讯作者：
Yeh, SL
中科院分区：
文献类型：
--
作者：
Hou, YC;Hsu, CS;Yeh, SL
本研究评价了谷氨酰胺（GLN）浓度是否与砷刺激的内皮细胞表面分子表达和多形核中性粒细胞（PMN）通过内皮细胞（EC）的迁移有关。人脐静脉内皮细胞（HUVECs）和中性粒细胞（PMNs）用不同浓度的GLN（0、300、600和1000 μ M）处理24小时。之后，我们用0.5 μ M砷刺激HUVECs 3小时，并允许PMNs迁移到ECs 2小时。检测HUVEC表面细胞粘附分子的表达及PMN表面整合素（CD 11b）和白细胞介素（IL）-8受体的表达。还分析了中性粒细胞的跨内皮迁移。结果表明，染砷组细胞粘附分子（CAM）和整合素表达均高于未染砷组。在砷组中，与其他GLN浓度相比，0 μ M时EC上的CAM和CD 11b以及PMN上的IL-8受体的表达最低。内皮细胞上的血管CAM-1和PMN表达上的CD 11b在300 μ M时高于600和1000 μ M GLN。300 μ M的EC和PMN分泌的IL-8高于600和1000 μ M的GLN，这与PMN上IL-8受体的表达一致。300 μ M GLN的多态性中性粒细胞迁移率显著高于其他GLN浓度。这些结果表明，砷刺激后，EC和PMN被激活。在GLN不存在的情况下，EC和PMN上的细胞粘附分子表达被抑制。与分解代谢条件相比，低GLN浓度导致更高的粘附分子表达和更大的中性粒细胞跨内皮迁移。谷氨酰胺管理水平接近或高于生理浓度降低IL-8和粘附分子的表达，中性粒细胞的迁移也减少砷刺激后。(c)2005年爱思唯尔公司All rights reserved.
This study evaluated whether glutamine (GLN)concentration was related to endothelial surface molecule expression and the migration of polymorphonuclear neutrophils (PMNs) through endothelial cells (ECs) stimulated by arsenic. Human umbilical vein endothelial cells (HUVECs) and PMNs were treated with different GLN concentrations (0, 300, 600 and 1000 mu M) for 24 h. After that, we stimulated HUVECs for 3 h with 0.5 mu M arsenic, and PMNs were allowed to transmigrate to ECs for 2 h. HUVEC surface expressions of cell adhesion molecules and integrin (CD11b) and interleukin (IL)-8 receptor expressions on PMNs were measured. The transendothelial migration of PMNs was also analyzed. The results showed that cell adhesion molecule (CAM) and integrin expressions in arsenic groups were higher than in those without arsenic. Among the arsenic groups, the expression of CAMs on ECs and CD11b, and IL-8 receptor on PMNs was lowest with 0 mu M compared with the other GLN concentrations. Vascular CAM-1 on ECs and CD11b on PMN expression were higher with 300 mu M than with 600 and 1000 mu M GLN. IL-8 secretions from ECs and PMNs were higher with 300 mu M than with 600 and 1000 mu M GLN, and this was consistent with the expression of the IL-8 receptor on PMNs. Polymorphonuclear neutrophil transmigration was significantly higher with 300 mu M GLN than with other GLN concentrations. These results suggest that ECs and PMNs were activated after arsenic stimulation. Cell adhesion molecule expressions on ECs and PMNs were suppressed in the absence of GLN. A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils. Glutamine administration at levels similar to or higher than physiological concentrations reduced IL-8 and adhesion molecule expression; PMN transmigration was also decreased after stimulation with arsenic. (c) 2005 Elsevier Inc. All rights reserved.