Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models
Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models
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DOI：
10.1117/1.jbo.18.12.126016
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发表时间：
2013-12-01
影响因子：
3.5
通讯作者：
Bouvet, Michael
中科院分区：
文献类型：
--
作者：
Maawy, Ali A.;Hiroshima, Yukihiko;Bouvet, Michael
本研究的目的是评估一组可见光和近红外染料共轭肿瘤特异性嵌合抗体的高分辨率肿瘤成像原位模型胰腺癌。将BxPC-3人胰腺癌原位植入裸鼠胰腺中。小鼠接受单次静脉内注射与以下荧光团之一缀合的嵌合抗癌胚抗原抗体：（Alexa Fluor 488或DyLight 488）; 550 nm组在一些实施方案中，所述荧光灯可以是荧光灯组（Alexa Fluor 555或DyLight 550）; 650-nm组（Alexa Fluor 660或DyLight 650）或750-nm组（Alexa Fluor 750或DyLight 755）。24小时后，使用Olympus OV 100小动物成像系统对小鼠进行非侵入性和活体荧光成像。比较了染料的成像深度、分辨率、肿瘤背景比（TBR）、光漂白和血红蛋白淬灭。较长波长的染料具有增加的穿透深度和检测最小肿瘤沉积物的能力，并提供最高的TBRs、对血红蛋白淬灭的抗性和特异性。较短波长的染料更耐光。该研究显示了每种染料在临床相关原位模型中用于特定癌症成像的独特优势。(C)作者。
The aim of this study was to evaluate a set of visible and near-infrared dyes conjugated to a tumor-specific chimeric antibody for high-resolution tumor imaging in orthotopic models of pancreatic cancer. BxPC-3 human pancreatic cancer was orthotopically implanted into pancreata of nude mice. Mice received a single intravenous injection of a chimeric anti-carcinoembryonic antigen antibody conjugated to one of the following fluorophores: 488-nm group (Alexa Fluor 488 or DyLight 488); 550-nm group (Alexa Fluor 555 or DyLight 550); 650-nm group (Alexa Fluor 660 or DyLight 650), or the 750-nm group (Alexa Fluor 750 or DyLight 755). After 24 h, the Olympus OV100 small-animal imaging system was used for noninvasive and intravital fluorescence imaging of mice. Dyes were compared with respect to depth of imaging, resolution, tumor-to-background ratio (TBR), photobleaching, and hemoglobin quenching. The longer wavelength dyes had increased depth of penetration and ability to detect the smallest tumor deposits and provided the highest TBRs, resistance to hemoglobin quenching, and specificity. The shorter wavelength dyes were more photostable. This study showed unique advantages of each dye for specific cancer imaging in a clinically relevant orthotopic model. (C) The Authors.