Steroid hormone 20-hydroxyecdysone promotes higher calcium mobilization to induce apoptosis
Steroid hormone 20-hydroxyecdysone promotes higher calcium mobilization to induce apoptosis
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类固醇激素 20-羟基蜕皮酮促进更高的钙动员以诱导细胞凋亡
DOI：
10.1016/j.ceca.2016.05.003
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发表时间：
2016-07-01
期刊：
影响因子：
4
通讯作者：
Zhao, Xiao-Fan
中科院分区：
文献类型：
--
作者：
Wang, Di;Pei, Xu-Yang;Zhao, Xiao-Fan
钙离子是调节多种细胞过程的重要二级信使，包括基因转录、细胞增殖和细胞凋亡。类固醇激素20-羟基蜕皮酮(20E)促进昆虫变态过程中的细胞程序性死亡，而保幼激素(OH)则抵消20E的活性以防止变态。20E和JH均可诱导细胞内钙离子浓度升高，但这两种拮抗激素引起细胞内钙离子浓度升高的机制和生理后果尚不清楚。在这里，我们使用棉铃虫表皮细胞系，我们发现20E通过G蛋白偶联受体诱导细胞内大量钙升高，而JH通过受体酪氨酸激酶诱导少量钙增加。钙释放激活的钙调节剂I(Grail)通道和瞬时受体电位(Trp)通道是20E诱导快速钙内流所必需的。20E处理后较长时间内维持较高的钙水平，ORAIL和Trp通道等基因的表达水平较JH处理后更高。20E可诱导Caspase3/7活化、细胞死亡和促凋亡基因表达，而JH不能诱导Caspase3/7活化。JH可抑制20E诱导的钙内流、caspase3/7激活和基因表达。较高的钙水平可诱导细胞凋亡。这些结果表明，20E和JH通过不同的途径在不同的水平上调节钙动员和动态平衡，从而导致不同的基因表达和细胞反应。(C)2016爱思唯尔有限公司。保留所有权利。
Calcium ions are essential secondary messengers that regulate diverse cellular processes including gene transcription, cell proliferation, and apoptosis. The steroid hormone 20-hydroxyecdysone (20E) promotes programmed cell death during insect metamorphosis, whereas juvenile hormone OH) counteracts 20E activity to prevent metamorphosis. Both 20E and JH can induce cellular calcium increase; however, the mechanisms and physiological consequences resulting from calcium increase caused by the two counteracting hormones are unclear. Here, using Helicoverpa armigera epidermal cell line, we show that 20E via a G-protein-coupled receptor induced a major calcium rise in the cells, whereas JH via receptor tyrosine kinase induced a minor calcium increase. The calcium release-activated calcium modulator I (Grail) and transient receptor potential (TRP) channels were necessary for 20E-induced rapid calcium influx. A higher calcium level was maintained in a long time and more genes including Orail and TRP channels showed elevated expression after the treatment of 20E than did after JH treatment. Caspase3/7 activation, cell death and pro-apoptotic gene expression were elicited by 20E induction, but not by JH. JH could repress 20E-induced calcium influx, caspase3/7 activation and gene expression. Higher calcium levels induced apoptosis. These results suggest that 20E and JH via different pathways regulate calcium mobilization and homeostasis at different levels, thus inform different gene expression and cellular responses. (C) 2016 Elsevier Ltd. All rights reserved.