Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: Molecular epidemiology and genome evolution
Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: Molecular epidemiology and genome evolution
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DOI：
10.1073/pnas.0307904100
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发表时间：
2004-02-24
影响因子：
11.1
通讯作者：
Chen, PJ
中科院分区：
文献类型：
--
作者：
Yeh, SH;Wang, HY;Chen, PJ
自2003年3月初以来，严重急性呼吸系统综合征（SARS）冠状病毒（CoV）感染已在台湾造成346例病例和37例死亡。这一流行病分两个阶段发生。第一阶段造成有限的家庭或医院感染，从3月初持续到4月中旬。所有病例均有明确的接触史，主要来自广东或香港。第二阶段则在市立医院爆发大规模疫情，并于4月下旬至6月中旬迅速蔓延到台湾北方和南部。在这一阶段，有一些零星的病例，无法追踪接触史。为了探讨台湾SARS冠状病毒的起源和传播途径，我们对10名SARS患者的病毒全基因组进行了系统的病毒谱系研究。台湾分离的SARS冠状病毒与广东、香港分离的SARS冠状病毒亲缘关系较近。此外，第二阶段的所有病例都属于市立医院爆发后的同一谱系，包括没有明显接触史的患者。对这些全长序列的分析表明，在SARS冠状病毒进化过程中发生了积极的选择。错配分布表明SARS病毒基因组没有达到平衡，并建议最近将病毒引入人群。估计的基因组突变率约为每个基因组0.1，这可能是已知RNA病毒中最低的突变率之一。
Since early March 2003, the severe acute respiratory syndrome (SARS) coronavirus (CoV) infection has claimed 346 cases and 37 deaths in Taiwan. The epidemic occurred in two stages. The first stage caused limited familial or hospital infections and lasted from early March to mid-April. All cases had clear contact histories, primarily from Guang-dong or Hong Kong. The second stage resulted in a large outbreak in a municipal hospital, and quickly spread to northern and southern Taiwan from late April to mid-June. During this stage, there were some sporadic cases with untraceable contact histories. To investigate the origin and transmission route of SARS-CoV in Taiwan's epidemic, we conducted a systematic viral lineage study by sequencing the entire viral genome from ten SARS patients. SARS-CoV viruses isolated from Taiwan were found closely related to those from Guang-dong and Hong Kong. in addition, all cases from the second stage belonged to the same lineage after the municipal hospital outbreak, including the patients without an apparent contact history. Analyses of these full-length sequences showed a positive selection occurring during SARS-CoV virus evolution. The mismatch distribution indicated that SARS viral genomes did not reach equilibrium and suggested a recent introduction of the viruses into human populations. The estimated genome mutation rate was approximate to0.1 per genome, demonstrating possibly one of the lowest rates among known RNA viruses.