Ultrasound augmenting injectable chemotaxis hydrogel for articular cartilage repair in osteoarthritis
Ultrasound augmenting injectable chemotaxis hydrogel for articular cartilage repair in osteoarthritis
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DOI：
10.1016/j.cclet.2020.12.004
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发表时间：
2021-05-04
影响因子：
9.1
通讯作者：
Qiu, Li
中科院分区：
文献类型：
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作者：
Liu, Hui;Xiang, Xi;Qiu, Li
骨性关节炎的发病率越来越高，严重影响了人们的生活质量，造成了巨大的社会经济负担。组织工程技术作为缓解骨性关节炎的关键治疗手段之一，已成为关节软骨修复领域的研究热点。水凝胶作为最常用的支架材料之一，可以为骨髓间充质干细胞等种子细胞提供良好的细胞外基质微环境，促进软骨再生。然而，干细胞的低归宿率严重限制了它们在促进关节软骨再生方面的作用。基质细胞衍生因子1α(SDF-1α)在骨髓间充质干细胞的活化、动员、归巢和迁移过程中起着至关重要的作用。因此，设计并制备了一种新型的可注射趋化水凝胶，该水凝胶由壳聚糖基可注射水凝胶和包埋SDF-1α的纳米微滴(PFP@NDS-PEG-SDF-1α)组成。然后利用超声波增强可注射的趋化水凝胶，促进骨髓间充质干细胞的归巢迁移，用于骨关节炎软骨修复。体外和体内实验证实，超声增强可注射的PFP@NDS-PEG-SDF-1α/水凝胶对BMSCs的迁移有明显的促进作用，显著促进了骨关节炎模型的干细胞归巢和软骨修复。因此，超声增强可注射趋化水凝胶的治疗策略在骨性关节炎关节软骨修复中具有广阔的应用前景。(C)中国化学学会、中国医学科学院药物研究所2021。爱思唯尔出版，版权所有。
The increasing incidence of osteoarthritis (OA) seriously affects life quality, posing a huge socioeconomic burden. Tissue engineering technology has become a hot topic in articular cartilage repair as one of the key treatment methods to alleviate OA. Hydrogel, one of the most commonly used scaffold materials, can provide a good extracellular matrix microenvironment for seed cells such as bone marrow mesenchymal stem cells (BMSCs), which can promote cartilage regeneration. However, the low homing rate of stem cells severely limits their role in promoting articular cartilage regeneration. Stromal cell-derived factor1 alpha (SDF-1 alpha) plays a crucial role in the activation, mobilization, homing, and migration of MSCs. Herein, a novel injectable chemotaxis hydrogel, composed of chitosan-based injectable hydrogel and embedding SDF-1 alpha-loaded nanodroplets (PFP@NDs-PEG-SDF-1 alpha) was designed and fabricated. The ultrasound was then used to augment the injectable chemotaxis hydrogel and promote the homing migration of BMSCs for OA cartilage repair. The effect of ultrasound augmenting injectable PFP@NDs-PEG-SDF-1 alpha/hydrogel on the migration of BMSCs was verified in vitro and in vivo, which remarkably promotes stem cell homing and the repair of cartilage in the OA model. Therefore, the treatment strategy of ultrasound augmenting injectable chemotaxis hydrogel has a bright potential for OA articular cartilage repair.(c) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.