Mechanism of hydrodynamic separation of biological objects in microchannel devices.
Mechanism of hydrodynamic separation of biological objects in microchannel devices.
复制标题
微通道装置中生物物体的流体动力分离机制。
作者：
Yu;C. Jen
在这项研究中，流体动力学色谱在微通道装置的分离机制进行了分析。这项工作的主要目的是提供一种方法来开发一个预测模型的流体动力学色谱的生物大分子在微通道中，并评估各种现象学系数的重要性。本文研究了微通道中颗粒流体动力学色谱的理论模型。一个充分发展的非反应性颗粒在微通道中的浓度分布，以阐明这些颗粒的流体动力学色谱。该模型考虑的作用在颗粒上的外力包括货车德瓦尔斯引力、双层力和重力。表面力，如货车德瓦尔斯吸引力以及双层排斥力，可以提高或阻碍高分子颗粒的平均速度。颗粒的平均速度随着分子半径的减小而减小，这是因为货车德瓦尔斯吸引力增加了通道表面附近的颗粒浓度，这是低速区域。颗粒的输运速度主要受重力的影响，密度越大，重力的影响越大。
In this study, the separation mechanism employed in hydrodynamic chromatography in microchannel devices is analyzed. The main purpose of this work is to provide a methodology to develop a predictive model for hydrodynamic chromatography for biological macromolecules in microchannels and to assess the importance of various phenomenological coefficients. A theoretical model for the hydrodynamic chromatography of particles in a microchannel is investigated herein. A fully developed concentration profile for non-reactive particles in a microchannel was obtained to elucidate the hydrodynamic chromatography of these particles. The external forces acting on the particles considered in this model include the van der Waals attractive force, double-layer force as well as the gravitational force. The surface forces, such as van der Waals attractive force as well as the double-layer repulsive force, can either enhance or hinder the average velocity of the macromolecular particles. The average velocity of the particles decreases with the molecular radius because the van der Waals attractive force increases the concentration of the particles near the channel surface, which is the low-velocity region. The transport velocity of the particles is dominated by the gravity and the higher density enlarges the effect caused by gravity.