Aggregation of Chlorophyll a Induced in Self-Assembled Membranes Composed of DMPC and DHPC
Aggregation of Chlorophyll a Induced in Self-Assembled Membranes Composed of DMPC and DHPC
复制标题
DMPC 和 DHPC 组成的自组装膜诱导叶绿素 a 的聚集
DOI：
10.1016/j.colsurfb.2018.12.008
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Hiroshi Umakoshi
中科院分区：
文献类型：
--
作者：
Shogo Taguchi;Keishi Suga;Keita Hayashi;Makoto Yoshimoto;Yukihiro Okamoto;Hidemi Nakamura;Hiroshi Umakoshi
叶绿素J聚集体在自然界中具有捕光天线的功能。在这项研究中，我们采用由长链1，2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）和短链1，2-二己酰-sn-甘油-3-磷酸胆碱（DHPC）组成的磷脂膜作为诱导Chla聚集体的平台。混合比（q）= 1.5的DMPC/DHPC组装体在20 °C下诱导Chlaat的J-聚集体，总脂质浓度为20 mM。而在q = ∞（DMPC囊泡）和q = 0（DHPC胶束）时，Chla聚集体在膜中未观察到。基于Soret带光谱（400-440 nm）的解卷积分析，估计DMPC/DHPC中q = 1.5的环境Chlamolecules包括较低极性的环境。ChlaJ-聚集体的光还原活性在较低的脂质浓度条件下进行了研究。
The J-aggregate of chlorophylla(Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chlaaggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chlaat 20 °C with a total lipid concentration of 20 mM. While, Chlaaggregates were not observed in the membranes atq= ∞ (DMPC vesicles) andq= 0 (DHPC micelles). The surroundings Chlamolecules in DMPC/DHPC atq= 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of ChlaJ-aggregates was investigated in lower lipid concentration conditions.