Structure and properties of mixed-chain phosphatidylcholine bilayers.
Structure and properties of mixed-chain phosphatidylcholine bilayers.
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混合链磷脂酰胆碱双层的结构和性质。
DOI：
10.1021/bi00469a030
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Shipley,GG
中科院分区：
文献类型：
--
作者：
Shah,J;Sripada,PK;Shipley,GG
摘要：用X射线衍射和差示扫描量热法研究了水合混合链磷脂酰胆碱（PC），C（8）：C（18）-PC和C（10）：C（18）-PC的结构和热致性质。对于完全水合的C（8）：C（18）-PC，在9.9 C（AH=13 kcal/mol）下观察到可逆的链熔融转变。在0 ℃（低于链熔融转变）的X射线衍射显示小的双层重复距离d=51.0和在4.1的尖锐的对称广角反射，这是混合的交叉双层凝胶相的特征[参见McIntosh，T. J.，西蒙，S.一、艾灵顿，J.C.，小的，&波特，N. A.（1984）Biochemistry 23，4038-4044; Hui，S. W.，梅森，J.T.，和黄，C.（1984）Biochemistry 23，5570-5577]。在30 ℃（高于链熔融转变）下，在4.5 A下观察到La相的特征扩散带，但具有增加的双层周期性，d= 61 A。计算的脂双层厚度（d）和直接从电子密度分布确定的脂双层厚度（
Revised Manuscript Received January 16, 1990 abstract: The structural and thermotropic properties of the hydrated mixed-chain phosphatidylcholines (PCs), C (8): C (18)-PC and C (10): C (18)-PC, have been studied by X-ray diffraction and differential scanning calorimetry. For fully hydrated C (8): C (18)-PC, the reversible chain melting transition is observed at 9.9 C (AH=13 kcal/mol). X-ray diffraction at 0 C (below the chain melting transition) shows a small bilayer repeat distance, d=51.0 A, and a sharp, symmetric wide-angle reflection at 4.1 A, characteristic of a mixed interdigitated bilayer gelphase [see McIntosh, T. J., Simon, S. A., Ellington, J. C., Jr., & Porter, N. A.(1984) Biochemistry 23, 4038-4044; Hui, S. W., Mason, J. T., & Huang, C.(1984) Biochemistry 23, 5570-5577]. At 30 C (above the chain melting transition), a diffuse band is observed at 4.5 A characteristic of an La phase but with an increased bilayer periodicity, d= 61 A. Both the calculated lipid bilayer thickness (d {) and that determined directly from electron density profiles (