Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles
Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles
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DOI：
10.1039/c9cc03277k
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发表时间：
2019-08-11
影响因子：
4.9
通讯作者：
Nomura，Shin-ichiro M.
中科院分区：
文献类型：
--
作者：
Sato，Yusuke;Komiya，Ken;Nomura，Shin-ichiro M.
在巨大的单层囊泡中实现了特异性DNA分子的等温扩增电路。使用此电路，超过5000倍的放大输出DNA的实现，和放大行为依赖于输入信号DNA的浓度在一个细胞大小的隔室。此外，证实了通过光刺激的扩增的起始。
An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.