DNA Binding and Cleavage Activity of Zinc(II) Complex of N,N′‐Bis(2‐guanidinoethyl)‐2,6‐pyridinedicarboxamide
DNA Binding and Cleavage Activity of Zinc(II) Complex of N,N′‐Bis(2‐guanidinoethyl)‐2,6‐pyridinedicarboxamide
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DOI：
10.1002/cjoc.200990290
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发表时间：
2009-09
影响因子：
5.4
通讯作者：
Y. Shao;Yong Ding;Weihong Xu;Li Wei;Fang Liu;Guoyuan Lu
中科院分区：
文献类型：
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作者：
Y. Shao;Yong Ding;Weihong Xu;Li Wei;Fang Liu;Guoyuan Lu
用圆二色谱和琼脂糖凝胶电泳研究了N，N′-二（胍基乙基）-2，6-吡啶二甲酰胺（Gua）锌配合物与DNA的相互作用。结果表明，Zn ~（2+）-Gua的DNA结合亲和力比Gua强，在生理条件下，Zn ~（2+）-Gua能促进超螺旋DNA的磷酸二酯键断裂，其断裂强度是DNA自然降解的106倍。Zn ~（2+）- Gua促进DNA断裂的可能机制为水解途径。加速是由于锌阳离子中心和官能团（双胍基）的协同催化。
The interaction of zinc(II) complex of N,N′-bis(guanidinoethyl)-2,6-pyridinedicarboxamide (Gua) with DNA was studied by CD spectroscopy and agarose gel electrophoresis analysis. The results indicate that the DNA binding affinity of Zn2+-Gua is stronger than that of Gua and the Zn2+-Gua can promote the cleavage of phosphodiester bond of supercoiled DNA under a physiological condition, which is ∼106 times higher than DNA natural degradation. The hydrolysis pathway was proposed as the possible mechanism for DNA cleavage promoted by the Zn2+- Gua. The acceleration is due to cooperative catalysis of the zinc cation center and the functional groups (bisguanidinium groups).