Over-expression of miR397 improves plant tolerance to cold stress in Arabidopsis thaliana
Over-expression of miR397 improves plant tolerance to cold stress in Arabidopsis thaliana
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DOI：
10.1007/s12374-013-0490-y
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发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
Pei, Haixia
中科院分区：
文献类型：
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作者：
Dong, Chun-Hai;Pei, Haixia
冷胁迫是限制植物地理分布和生长季节的环境因子。植物对低温的反应涉及不同的代谢途径和基因调控。尽管一些miRNAs的表达在低温胁迫下发生了显著的变化，但miRNAs在植物抗寒性中的调控功能还知之甚少。在这项研究中，我们产生了转基因拟南芥植物，其中含有过表达的35 S：：miR 397 a的构建体。通过RNA印迹分析揭示了转化植物中高水平的miR 397 a转录物。植物耐冷性测定表明，miR 397 a的过表达提高了植物对低温和冷冻胁迫的耐受性。此外，该研究表明，miR 397 a-ov植物的抗冻性通过在4A ℃下的冷驯化而提高。北方印迹分析显示miR 397 a的过表达影响了CBF冷调节基因和下游COR基因的表达。这些发现提供了证明miR 397在冷信号传导途径和植物对低温和冷冻胁迫的耐受性中的调节作用的证据。
Cold stress is an environmental factor that limits the geographical distribution and growing season of plants. The plant response to cold involves different metabolic pathways and gene regulation. Although the expression of some miRNAs is significantly altered in cold stress, little is known about the regulatory function of miRNAs in plant resistance to cold. In this study, we generated transgenic Arabidopsis plants which harbor an over-expression construct of 35S::miR397a. Analysis by RNA blotting revealed high levels of miR397a transcripts in the transformed plants. Plant cold tolerance assays demonstrated that over-expression of miR397a improved plant tolerance to chilling and freezing stresses. In addition, this study showed that the freezing tolerance of miR397a-ov plants was improved by cold acclimation at 4A degrees C. Northern blot analysis revealed that overexpression of miR397a affected the expression of coldregulated CBF genes and downstream COR genes. These findings provide evidence demonstrating a regulatory role of miR397 in the cold signaling pathway and plant tolerance to chilling and freezing stresses.