Release of Moxifloxacin From Corneal Collagen Shields.
Release of Moxifloxacin From Corneal Collagen Shields.
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莫西沙星从角膜胶原盾的释放。
DOI：
10.1097/icl.0000000000000421
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Shanks,RobertMQ
中科院分区：
文献类型：
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作者：
Zhou,Siwei;Hunt,KristinM;Grewal,ArmanS;Brothers,KimberlyM;Dhaliwal,DeepinderK;Shanks,RobertMQ
目的：探讨莫西沙星在角膜绷带接触镜（bcl）和角膜胶原膜（CSs）中的扩散，bcl和角膜胶原膜释放莫西沙星的相对能力，以及临床环境中莫西沙星从角膜接触镜释放的潜力。方法：采用体外模型，比较5%莫西沙星在bcl和CSs中的扩散。其次，测定0.5%莫西沙星浸泡bcl和CSs的药物释放量。最后，根据临床模型，将CSs浸泡在Vigamox（市售莫西沙星）中，检测总释放浓度。胶原蛋白保护层在24小时后保持完整；因此，对CS进行酶解和机械研磨，以确定是否能进一步释放药物。用分光光度计在设定的时间点测量莫西沙星的浓度，直至24小时。结果：在扩散实验中，35.7±10.5%的细胞通过bcl扩散，36.2±11.8%的细胞通过CSs扩散（P= 0.77）。吸收实验表明，在120 min时，bcl的释放量为33.3±6.77 μg/mL，而CSs的释放量为45.8±5.2 μg/mL （P= 0.0008）。体外模拟维加莫西浸渍CS的临床应用实验发现，2ml磷酸盐缓冲盐水24h释放莫西沙星浓度为127.7±7.25 μg/mL。结论：莫西沙星在bcl和CSs中的扩散率相似；然而，与bcl相比，cs具有更大的吸收和释放莫西沙星的能力。维加莫注射液可释放莫西沙星250 μg，可能是预防眼内炎的有效方法。
Objectives:To investigate the diffusion of moxifloxacin through bandage contact lenses (BCLs) versus corneal collagen shields (CSs), the relative ability of BCLs and CSs to release moxifloxacin, and the potential of release of moxifloxacin from CSs in the clinical setting.Methods:Using an in vitro model, the diffusion of 5% moxifloxacin across BCLs and CSs was compared. Next, the amount of drug release from BCLs and CSs soaked in 0.5% moxifloxacin was measured. Finally, based on a clinical model, CSs were soaked in Vigamox (commercial moxifloxacin) and the total concentration released was detected. Collagen shields remained intact after 24 hr; therefore, enzymatic digestion and mechanical grinding of the CS were performed to determine whether further drug could be released. The concentration of moxifloxacin was measured using a spectrophotometer at set time points up to 24 hr.Results:In the diffusion assay, 35.7±10.5% diffused through the BCLs and 36.2±11.8% diffused through the CSs (P= 0.77). The absorption assay demonstrated at 120 min, a total of 33.3±6.77 μg/mL was released from BCLs compared with 45.8±5.2 μg/mL from the CSs (P= 0.0008). In vitro experiments to simulate clinical application of Vigamox-soaked CS found the concentration of moxifloxacin released of 127.7±7.25 μg/mL in 2 mL of phosphate-buffered saline over 24 hr.Conclusions:Moxifloxacin diffuses through BCLs and CSs at similar rates; however, CSs have greater capacity to absorb and release moxifloxacin compared with BCLs. Vigamox-soaked CSs released 250 μg of moxifloxacin and may be a useful method to prevent endophthalmitis.