The E3 ubiquitin ligase DESYNAPSIS1 regulates synapsis and recombination in rice meiosis
The E3 ubiquitin ligase DESYNAPSIS1 regulates synapsis and recombination in rice meiosis
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DOI：
10.1016/j.celrep.2021.109941
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发表时间：
2021-11-02
期刊：
影响因子：
8.8
通讯作者：
Cheng，Zhukuan
中科院分区：
文献类型：
--
作者：
Ren，Lijun;Zhao，Tingting;Cheng，Zhukuan
联会复合体（SC）的组装和同源重组是减数分裂前期最关键的事件，是减数分裂染色体可靠分离的前提。然而，基本的监管机制在很大程度上仍然未知。在这里，我们揭示了一个功能性的环指E3泛素连接酶，DESYNAPSIS1（DSNP1），在水稻减数分裂过程中的SC组装和同源重组中起着重要的作用。在dsnp1突变体中，同源突触是不连续的，异常的SC样多复合体的发生独立于同源染色体的共对齐。随着减数分裂染色体上HEI10、ZIP4和MER3位点的减少，snp1减数分裂母细胞的交换数（CO）也急剧减少。此外，中心元件的缺失在很大程度上恢复了非ZEP 1 ZMM蛋白的定位和dsnp 1背景中CO的数量。总的来说，DSNP 1沿着成对的同源染色体沿着稳定了典型的三重SC结构，并进一步促进了CO的形成。
Synaptonemal complex (SC) assembly and homologous recombination, the most critical events during prophase I, are the prerequisite for faithful meiotic chromosome segregation. However, the underlying regulatory mechanism remains largely unknown. Here, we reveal that a functional RING finger E3 ubiquitin ligase, DESYNAPSIS1 (DSNP1), plays significant roles in SC assembly and homologous recombination during rice meiosis. In thedsnp1mutant, homologous synapsis is discontinuous and aberrant SC-like polycomplexes occur independent of coaligned homologous chromosomes. Accompanying the decreased foci of HEI10, ZIP4, and MER3 on meiotic chromosomes, the number of crossovers (COs) decreases dramatically indsnp1meiocytes. Furthermore, the absence of central elements largely restores the localization of non-ZEP1 ZMM proteins and the number of COs in thedsnp1background. Collectively, DSNP1 stabilizes the canonical tripartite SC structure along paired homologous chromosomes and further promotes the formation of COs.