Growth hormone regulates intestinal gene expression of nutrient transporters in tilapia (Oreochromis mossambicus)
Growth hormone regulates intestinal gene expression of nutrient transporters in tilapia (Oreochromis mossambicus)
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DOI：
10.1016/j.ygcen.2020.113464
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发表时间：
2020-06-01
影响因子：
2.7
通讯作者：
Seale, Andre P.
中科院分区：
文献类型：
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作者：
Petro-Sakuma, Cody;Celino-Brady, Fritzie T.;Seale, Andre P.
生长激素（GH）是硬骨鱼生长生理的基础，它通过多种途径促进营养物质在肠上皮细胞中的吸收。当前的研究探讨了GH对杂食性硬骨鱼莫桑比克罗非鱼（Oreochromis mossambicus）营养转运蛋白基因表达的影响。我们采用脑垂体切除术（垂体切除术）和激素替代治疗，以评估是否GH指导基因表达的GH受体（GHR 2），肽转运蛋白，pept 1a，pept 1b和pept 2，氨基酸转运蛋白，slc 7a 9，Na+/葡萄糖协同转运蛋白，sglt 1，葡萄糖转运蛋白，sglt 2，和肌醇转运蛋白，smit 2，在前，中，后肠。ghr 2主要在后肠中表达，而peptla、pept 1b、slc 7a 9、sglt 1、glt 2和smit 2在前肠和/或中肠中表现出最高的mRNA水平。虽然垂体切除罗非鱼表现出减少表达的ghr 2，pept 1a，pept 1b，slc 7a 9，和pep 2与完整的和假手术的控制相比，只有ghr 2，pept 1a，pept 1b和pep 2水平恢复生长激素替代。我们的研究结果表明，生长激素支持的增长，至少在一定程度上，通过刺激其同源受体的基因表达和肠道中的关键营养转运蛋白。
Among the various ways that growth hormone (GH) underlies the growth physiology of teleost fishes, GH stimulates transport pathways that facilitate the absorption of nutrients across intestinal epithelia. The current study investigated the effects of GH on the gene expression of nutrient transporters in an omnivorous teleost, the Mozambique tilapia (Oreochromis mossambicus). We employed pituitary gland removal (hypophysectomy) and hormone replacement to assess whether GH directs the gene expression of the GH receptor (ghr2), the peptide transporters, pept1a, pept1b and pept2, the amino acid transporter, slc7a9, the Na+/glucose cotransporter, sglt1, the glucose transporter, glut2, and the myo-inositol transporter, smit2, in anterior, middle, and posterior intestine. ghr2 was predominantly expressed in posterior intestine, while peptla, pept1b, slc7a9, sglt1, glut2, and smit2 exhibited the highest mRNA levels in anterior and/or middle intestine. While hypophysectomized tilapia exhibited diminished expression of ghr2, pept1a, pept1b, slc7a9, and glut2 compared with intact and sham-operated controls, only ghr2, pept1a, pept1b and glut2 levels were restored by GH replacement. Our findings indicate that GH supports growth, at least in part, by stimulating the gene expression of its cognate receptor and key nutrient transporters in the intestine.