Effects of a Commercial Microbial Agent on the Bacterial Communities in Shrimp Culture System.
Effects of a Commercial Microbial Agent on the Bacterial Communities in Shrimp Culture System.
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商业微生物剂对虾培养系统中细菌群落的影响。
DOI：
10.3389/fmicb.2018.02430
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Liu Z;Qiuqian L;Yao Z;Wang X;Huang L;Zheng J;Wang K;Li L;Zhang D
商业微生物剂（例如，益生菌、微生物制品、微生物制剂等）已广泛应用于对虾养殖病害防治。然而，这些微生物制剂（MA）对对虾健康的影响是不稳定的，其潜在的机制仍然不清楚。MA的效果可能是通过影响对虾养殖系统的细菌群落来实现的。为了验证这一假设，我们使用16S rRNA基因扩增子测序，以调查在虾养殖池塘中，有或没有商业MA每周应用的肠道细菌组成的动态。结果表明，施用MA提高了浮游细菌群落的时间周转率。在1周内，MA处理显着驱动浮游细菌群落组成，从没有MA处理后第2天MA应用程序，但偏离趋于消失，在第4和第7天。第21天，两组对虾肠道细菌群落差异显著。甲基丙烯酸处理组虾肠中杜鹃花科的相对丰度显著高于对照组。然而，MA处理并没有显着提高虾的生长或存活率。本研究表明，微生态制剂能促进浮游细菌群落的更新和肠道细菌群落的迁移，但其对对虾生长的影响差异较大，可通过优化微生态制剂的激活和应用方法来改善，因此，在开发和应用微生态制剂之前，需要对对虾养殖系统的微生态过程进行更多的研究。
Commercial microbial agents (e.g., probiotics, microbial products, microorganism preparation et al.) have been widely applied for disease control in shrimp culture. However, the effect of these microbial agents (MA) on shrimp health is unstable and the underlying mechanism remains unclear. The effect of MA can probably be achieved by influencing the bacterial community of shrimp culture system. To test this hypothesis, we used 16S rRNA gene amplicon sequencing to investigate the dynamics of both planktonic and intestinal bacterial composition in shrimp culture ponds with or without commercial MA applied weekly. The results showed that MA application increased the temporal turnover rate of bacterioplankton community. Within 1 week, MA-treatment significantly drove bacterioplankton community composition to divert from that without MA-treatment at day 2 after MA application, but the deviation tended to vanish at days 4 and 7. At day 21, a significant difference was observed in shrimp intestinal bacterial community between two groups. The relative abundance of Rhodobacteraceae in shrimp intestine was significantly greater in the MA-treated group than that in the control. However, MA-treatment did not significantly improve the growth or survival ratio of shrimp. This study suggest that MA works in terms of accelerating bacterioplankton community turnover and shifting intestinal bacterial community, however, its effect on shrimp growth might vary greatly and might be improved by optimizing the method in activation and application and more investigation on the microbial ecological process of shrimp culture system is needed before we develop and apply probiotics more efficiently.
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DOI：
10.1038/ismej.2012.56
发表时间：
2012-12
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
用于鲶鱼病原体生物防治的芽孢杆菌菌株的鉴定。
影响因子：
3.7
作者：
Ran C;Carrias A;Williams MA;Capps N;Dan BC;Newton JC;Kloepper JW;Ooi EL;Browdy CL;Terhune JS;Liles MR
通讯作者：
Liles MR
影响因子：
3.7
作者：
Sim K;Cox MJ;Wopereis H;Martin R;Knol J;Li MS;Cookson WO;Moffatt MF;Kroll JS
通讯作者：
Kroll JS
影响因子：
3.5
作者：
Boonthai, T.;Vuthiphandchai, V.;Nimrat, S.
通讯作者：
Nimrat, S.
影响因子：
6.9
作者：
Ma, Zhen;Song, Xiefa;Gao, Lei
通讯作者：
Gao, Lei