Expression of the proto-oncoprotein breast cancer nuclear receptor auxiliary factor (Brx) is altered in eutopic endometrium of women with endometriosis
Expression of the proto-oncoprotein breast cancer nuclear receptor auxiliary factor (Brx) is altered in eutopic endometrium of women with endometriosis
复制标题
DOI：
10.1016/j.fertnstert.2005.06.053
复制
发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
Nieman, L
中科院分区：
文献类型：
--
作者：
Hearns-Stokes, R;Mayers, C;Nieman, L
目的：探讨雌激素受体α (ER α)、雌激素受体β (ER β)和乳腺癌核受体辅助因子（Brx）在正常女性和子宫内膜异位症患者异位子宫内膜中的表达。设计：前瞻性观察研究。环境：三级医疗和研究中心。患者：29名子宫内膜异位症患者和35名年龄相近的健康排卵志愿者。干预措施：子宫内膜活检。主要观察指标：月经周期异位子宫内膜中ER α、ER β和Brx蛋白的免疫组化染色强度和定位。结果：ER - α和ER - β的表达在增殖期最高，两组差异无统计学意义。Brx的表达在健康志愿者和子宫内膜异位症患者之间存在差异。在增殖期，与健康志愿者相比，子宫内膜异位症患者活检组织的腺体和间质室中Brx的免疫染色强度都更高；核基质Brx染色在子宫内膜异位症患者中更为常见。结论：子宫内膜异位症患者异位子宫内膜中Brx的时空表达发生改变。这些发现表明子宫内膜异位症患者的子宫内膜发生了根本性的改变。Brx在子宫内膜异位着床中的作用值得进一步研究。
Objective: To evaluate the expression of estrogen receptor alpha (ER alpha), estrogen receptor beta (ER beta), and breast cancer nuclear receptor auxiliary factor (Brx) in eutopic endometrium of normal women and women with endometriosis.Design: Prospective observational study.Setting: Tertiary care and research center.Patient(s): Twenty-nine women with endometriosis and 35 healthy ovulatory volunteers of similar ages.Intervention(s): Endometrial biopsy.Main Outcome Measure(s): Expression of immunohistochemical staining intensity and localization of ER alpha, ER beta, and Brx proteins in eutopic endometrium during the menstrual cycle.Result(s): Expression of ER alpha and ER beta was highest in the proliferative phase and was similar in both groups. Brx expression differed between healthy volunteers and those with endometriosis. During the proliferative phase, immunostaining intensity of Brx was greater in both the glandular and the stromal compartments of biopsies from patients with endometriosis compared to healthy volunteers; nuclear stromal Brx staining was more common in patients with endometriosis.Conclusion(s): The spatiotemporal expression of Brx was altered in eutopic endometrium of women with endometriosis. These findings suggest a fundamental alteration in the endometrium of women who have endometriosis. The role of Brx in ectopic implantation of endometrium deserves further study.