Low-intensity pulsed ultrasound inhibits adipogenic differentiation via HDAC1 signalling in rat visceral preadipocytes.
Low-intensity pulsed ultrasound inhibits adipogenic differentiation via HDAC1 signalling in rat visceral preadipocytes.
复制标题
低强度脉冲超声通过大鼠内脏前脂肪细胞中的 HDAC1 信号传导抑制脂肪形成分化。
DOI：
10.1080/21623945.2019.1643188
复制
发表时间：
2019-12-01
期刊：
影响因子：
3.3
通讯作者：
Kong, Xiangqing
中科院分区：
文献类型：
--
作者：
Xu, Tianhua;Zhao, Kun;Kong, Xiangqing
针对脂肪细胞分化的非药物策略对于缓解内脏性肥胖及其相关疾病至关重要。然而，低强度脉冲超声（LIPUS）是否以及如何用于抑制内脏脂肪细胞分化尚不完全清楚。在本研究中，我们旨在研究LIPUS对原发性内脏前脂肪细胞分化的影响及其相关机制，并探讨其在临床内脏肥胖治疗中的潜在作用。将前脂肪细胞每天暴露于LIPUS （0.5 MHz, 1.2 MPa）中10分钟。通过脂滴的形成、成脂转录因子和代表性标志物的水平来估计成脂分化。western blotting检测丝裂原活化蛋白激酶（MAPK）成员蛋白和组蛋白乙酰化相关分子。平均声压为1.2 MPa的LIPUS刺激可显著抑制成脂分化和成脂标志物的表达。作为一种机制，LIPUS治疗增加了组蛋白去乙酰化酶1 （HDAC1）的核水平，降低了组蛋白3和组蛋白4的乙酰化。同时，抑制HDAC1可通过提高AcH3和AcH4水平，阻断LIPUS对成脂分化的抑制作用。我们的研究可能为临床内脏性肥胖控制提供一种基于超声的有希望的策略。
Non-drug strategy targeting adipocyte differentiation is critical for alleviating visceral obesity and its related diseases. However, whether and how low intensity pulsed ultrasound (LIPUS) could be used for inhibiting visceral adipocyte differentiation is not fully understood. In this study, we aim to investigate the effect and associated mechanism of LIPUS on primary visceral preadipocyte differentiation and explore its potential role for clinical visceral obesity management. The preadipocytes were daily exposed to LIPUS (0.5 MHz, 1.2 MPa) for 10 min. Adipogenic differentiation was estimated by the formation of lipid droplets and the levels of adipogenic transcriptional factors and representative markers. Mitogen-activated protein kinase (MAPK) member proteins and histone acetylation-related molecules were measured by western blotting. LIPUS stimulation with an average acoustic pressure of 1.2 MPa led to a prominent inhibition of adipogenic differentiation and expression of adipogenic markers. As a mechanism, LIPUS treatment increased the nuclear levels of histone deacetylase 1 (HDAC1) and decreased the acetylation of histone 3 and histone 4. Meanwhile, the inhibition of the HDAC1 could block the inhibitory effect of LIPUS on adipogenic differentiation via increasing AcH3 and AcH4 levels. Our study may provide an ultrasound-based promising strategy for clinical visceral obesity control.