DETECTION AND MAPPING OF AMPLIFIED DNA-SEQUENCES IN BREAST-CANCER BY COMPARATIVE GENOMIC HYBRIDIZATION
DETECTION AND MAPPING OF AMPLIFIED DNA-SEQUENCES IN BREAST-CANCER BY COMPARATIVE GENOMIC HYBRIDIZATION
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DOI：
10.1073/pnas.91.6.2156
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发表时间：
1994-03-15
影响因子：
11.1
通讯作者：
WALDMAN, FM
中科院分区：
文献类型：
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作者：
KALLIONIEMI, A;KALLIONIEMI, OP;WALDMAN, FM
比较基因组杂交应用于5个乳腺癌细胞系和33个原发性肿瘤，以发现和映射具有增加的DNA序列拷贝数的基因组区域。三分之二的原发性肿瘤和几乎所有的细胞系都表现出DNA序列拷贝数增加，共影响26个染色体亚区。这些基因座中的大多数与目前已知的乳腺癌扩增基因不同，其中17 q22-q24和20 q13的序列显示出最高的扩增频率。结果表明，这些染色体区域可能包含以前未知的基因，其表达增加有助于乳腺癌的进展。拷贝数增加的染色体区域通常跨越数十Mb，表明每个区域中涉及多个基因。
Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors to discover and map regions of the genome with increased DNA-sequence copy-number. Two-thirds of primary tumors and almost all cell lines showed increased DNA-sequence copy-number affecting a total of 26 chromosomal subregions. Most of these loci were distinct from those of currently known amplified genes in breast cancer, with sequences originating from 17q22-q24 and 20q13 showing the highest frequency of amplification. The results indicate that these chromosomal regions may contain previously unknown genes whose increased expression contributes to breast cancer progression. Chromosomal regions with increased copy-number often spanned tens of Mb, suggesting involvement of more than one gene in each region.