pdh modulate virulence through reducing stress tolerance and biofilm formation of Streptococcus suis serotype 2
pdh modulate virulence through reducing stress tolerance and biofilm formation of Streptococcus suis serotype 2
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pdh 通过降低猪链球菌血清型 2 的应激耐受性和生物膜形成来调节毒力。
DOI：
10.1080/21505594.2019.1631661
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发表时间：
2019-01-01
期刊：
影响因子：
5.2
通讯作者：
Yi, Li
中科院分区：
文献类型：
--
作者：
Wang, Yang;Wang, Yuxin;Yi, Li
猪链球菌血清型 2 (S. suis 2) 是一种人畜共患病原体。它引起猪脑膜炎、关节炎、肺炎和败血症，导致极高的死亡率，严重影响公众健康和养猪业的发展。丙酮酸脱氢酶(PDH)是一种重要的糖代谢酶，广泛存在于微生物、哺乳动物和高等植物中。它催化丙酮酸不可逆氧化脱羧为乙酰辅酶A，并将 NAD+ 还原为 NADH。在本研究中，我们发现在小鼠感染模型中，猪链球菌ZY05719序列7型pdh缺失株（pdh）的毒力显着低于野生型株（WT）。感染pdh的小鼠血液和器官中活菌的分布明显低于感染WT的小鼠。温度应激、盐应激和氧化应激导致细菌存活率降低。此外，与WT相比，pdh的粘附和侵袭PK15细胞的能力、生物膜形成和抗应激能力均显着降低。此外，实时PCR结果表明pdh缺失降低了多种粘附相关基因的表达。然而，互补菌株（Cpdh）和WT之间的相关性生物学分析没有显着差异。而且，RAW264.7巨噬细胞中pdh的存活率显着低于WT株。本研究表明PDH参与了猪链球菌2的发病机制，PDH毒力降低可能与菌株抵抗应激能力下降有关。
Streptococcus suis serotype 2 (S. suis 2) is a zoonotic pathogen. It causes meningitis, arthritis, pneumonia and sepsis in pigs, leading to extremely high mortality, which seriously affects public health and the development of the pig industry. Pyruvate dehydrogenase (PDH) is an important sugar metabolism enzyme that is widely present in microorganisms, mammals and higher plants. It catalyzes the irreversible oxidative decarboxylation of pyruvate to acetyl-CoA and reduces NAD+ to NADH. In this study, we found that the virulence of the S. suis ZY05719 sequence type 7 pdh deletion strain (pdh) was significantly lower than the wild-type strain (WT) in the mouse infection model. The distribution of viable bacteria in the blood and organs of mice infected with the pdh was significantly lower than those infected with WT. Bacterial survival rates were reduced in response to temperature stress, salt stress and oxidative stress. Additionally, compared to WT, the ability to adhere to and invade PK15 cells, biofilm formation and stress resistance of pdh were significantly reduced. Moreover, real-time PCR results showed that pdh deletion reduced the expression of multiple adhesion-related genes. However, there was no significant difference in the correlation biological analysis between the complemented strain (Cpdh) and WT. Moreover, the survival rate of pdh in RAW264.7 macrophages was significantly lower than that of the WT strain. This study shows that PDH is involved in the pathogenesis of S. suis 2 and reduction in virulence of pdh may be related to the decreased ability to resist stress of the strain.