Role of nutrient concentrations and water movement on diatom's productivity in culture
Role of nutrient concentrations and water movement on diatom's productivity in culture
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DOI：
10.1038/s41598-018-37611-6
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发表时间：
2019-02-06
影响因子：
4.6
通讯作者：
Brunet, Christophe
中科院分区：
文献类型：
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作者：
Orefice, Ida;Musella, Margherita;Brunet, Christophe
微藻生长最大化正在成为增强这些光合作用微生物的生物技术命运的一项责任。这项研究基于大量的数据，旨在重新审视硅藻的实验室培养，目的是提高硅藻的生长速度和生物量。本文研究了实验室生长的沿海硅藻Marinoi Sarno&Zingone在常规f/2曝气和两种改良条件下的生长能力和对资源的需求。结果表明，通过将磷酸盐、硅酸盐、微量元素和维生素的浓度加倍，成功地提高了生长速度，防止了f/2培养基中磷酸盐或硅酸盐的限制。然而，与曝气相比，无论在哪种介质(f/2或富营养液)中，水分运动都会导致生长效率的提高，从而影响硅藻对营养物质的需求和消耗。这项研究是提高硅藻生物量生产，降低其成本的重要一步，符合蓝色生物技术的要求。
Microalgal growth maximization is becoming a duty for enhancing the biotechnological fate of these photosynthetic microorganisms. This study, based on an extensive set of data, aims to revisit diatom's cultivation in laboratory with the objective to increase growth rate and biomass production. We investigated the growth ability and resource requirements of the coastal diatom Skeletonema marinoi Sarno & Zingone grown in laboratory in the conventional f/2 medium with aeration and in two modified conditions: (i) the same medium with water movement inside and (ii) an enriched medium with the same water movement. Results revealed that, by doubling the concentration of phosphate, silicate, microelements and vitamins, growth rate was successfully enhanced, preventing phosphate or silicate limitation in the f/2 culture medium. Yet, irrespective of the media (f/2 or enriched one), water movement induced an increase of growth efficiency compared to aeration, affecting nutrients' requirement and consumption by diatoms. This study is an important step for enhancing diatom biomass production, reducing its cost, as required in the blue biotechnology context.