Taraxacum official (dandelion) leaf extract alleviates high-fat diet-induced nonalcoholic fatty liver
Taraxacum official (dandelion) leaf extract alleviates high-fat diet-induced nonalcoholic fatty liver
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DOI：
10.1016/j.fct.2013.04.023
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发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
Sung, Mi Jeong
中科院分区：
文献类型：
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作者：
Davaatseren, Munkhtugs;Hur, Haeng Jeon;Sung, Mi Jeong
本研究旨在探讨蒲公英叶提取物（DLE）对高脂肪日粮（HFD）诱导的肝脏脂肪变性的保护作用，并阐明其分子机制。为了研究DLE对C57BL/6小鼠的肝保护作用，我们给C57BL/6小鼠喂食正常饲料（NCD）、高脂饲料（HFD）、高脂饲料中添加2 g/kg DLE DLE （DL）和HFD中添加5 g/kg DLE （DH）。我们发现，与单独使用HFD相比，添加DLE的HFD显著减少了肝脏脂质积累。DL和DH组的体重和肝脏重量显著低于HFD组，添加DLE可显著抑制HFD诱导的甘油三酯（TG）、总胆固醇（TC）、胰岛素、血清空腹血糖水平和稳态模型评估胰岛素抵抗（HOMA-IR）。此外，DLE处理显著增加了肝脏和肌肉蛋白中腺苷单磷酸（AMP）活化蛋白激酶（AMPK）的活化。DLE通过AMPK途径显著抑制hfd喂养的C57BL/6小鼠肝脏脂质积累，降低胰岛素抵抗和脂质水平。这些结果表明，DLE可能是预防和治疗肥胖相关的非酒精性脂肪性肝病的一种有希望的方法。(C) 2013 Elsevier Ltd.版权所有。
The purpose of this study is to determine the protective effect of Taraxacum official (dandelion) leaf extract (DLE) on high-fat-diet (HFD)-induced hepatic steatosis, and elucidate the molecular mechanisms behind its effects. To determine the hepatoprotective effect of DLE, we fed C57BL/6 mice with normal chow diet (NCD), high-fat diet (HFD), HFD supplemented with 2 g/kg DLE DLE (DL), and HFD supplemented with 5 g/kg DLE (DH). We found that the HFD supplemented by DLE dramatically reduced hepatic lipid accumulation compared to HFD alone. Body and liver weights of the DL and DH groups were significantly lesser than those of the HFD group, and DLE supplementation dramatically suppressed triglyceride (TG), total cholesterol (TC), insulin, fasting glucose level in serum, and Homeostatic Model Assessment Insulin Resistance (HOMA-IR) induced by HFD. In addition, DLE treatment significantly increased activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) in liver and muscle protein. DLE significantly suppressed lipid accumulation in the liver, reduced insulin resistance, and lipid in HFD-fed C57BL/6 mice via the AMPK pathway. These results indicate that the DLE may represent a promising approach for the prevention and treatment of obesity-related nonalcoholic fatty liver disease. (C) 2013 Elsevier Ltd. All rights reserved.