Hydrogel-Involved Colorimetric Platforms Based on Layered Double Oxide Nanozymes for Point-of-Care Detection of Liver-Related Biomarkers
Hydrogel-Involved Colorimetric Platforms Based on Layered Double Oxide Nanozymes for Point-of-Care Detection of Liver-Related Biomarkers
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DOI：
10.1021/acsami.1c21578
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发表时间：
2022-01-26
影响因子：
9.5
通讯作者：
Li, Yingchun
中科院分区：
文献类型：
--
作者：
Liu, Xiaoxue;Mei, Xuecui;Li, Yingchun
方便、低成本地监测肝脏状态对于及时预警药物性肝病具有重要意义。在此，我们设计了一种新的比色床旁（POC）平台，用于测定三种肝脏相关生物标志物：天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）。该平台将琼脂糖水凝胶集成到便携式设备中，其中水凝胶装载有纳米酶和不同的反应物质，用于触发特定反应并产生比色信号。典型地，Au修饰的CoAl层状双氧化物（Au/LDO）首次被开发为具有过氧化物酶（POD）模拟活性的纳米酶，其可以在过氧化氢（H2 O2）共存下加速无色的3，3 '，5，5'-四甲基联苯胺（TMB）氧化为蓝色oxTMB。AST和ALT的检测机制是基于它们可以引起单独的级联反应产生H2 O2，H2 O2进一步激活Au/LDO纳米酶催化TMB的显色反应。碱性磷酸酶能催化L-抗坏血酸-2-磷酸水解生成抗坏血酸。后者然后使在Au/LDO的辅助下产生的oxTMB变色。与智能手机配合使用，水凝胶的颜色信息可以转换为色调值，从而可以对ALT、AST和ALP进行定量分析，检测限分别为15、10和5 U/L。该方法简单、成本低，可用于人血浆中三种分析物的同时测定。此外，由于水凝胶是一次性的，并且可以被装载有不同反应试剂的新水凝胶替换，因此该平台有望用于各种化学/生物靶标的POC测试。
Monitoring the liver status in a convenient and low-cost way is significant for obtaining a warning about drug-indued liver diseases promptly. Herein, we designed a novel colorimetric point-of-care (POC) platform for the determination of three liver-related biomarkers.aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). This platform integrated agarose hydrogels into a portable device, where hydrogels were loaded with nanozymes and different reaction substances for triggering specific reactions and generating colorimetric signals. Typically, Au-decorated CoAl-layered double oxide (Au/LDO) was for the first time developed as the nanozyme with peroxidase (POD) mimic activity, which can accelerate the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxTMB with the coexistence of hydrogen peroxide (H2O2). The detection mechanism of AST and ALT is based on the fact that they can cause individual cascade reactions to generate H2O2, and H2O2 further activates the Au/LDO nanozyme to catalyze the chromogenic reaction of TMB. As for ALP, it can catalytically hydrolyze L-ascorbic acid-2-phosphate to ascorbic acid. The latter then discolored the oxTMB that was produced with the assistance of Au/LDO. Teaming up with a smartphone, the color information of hydrogels can be converted to hue values, which allow quantitative analysis of ALT, AST, and ALP with detection limits of 15, 10, and 5 U/L, respectively. Moreover, the simple and cost-effective platform was successfully applied for the simultaneous determination of the three analytes in human plasma. Additionally, since the hydrogel is disposable and can be replaced by new ones loaded with different reaction regents, the platform is expected to serve the POC testing of various chem/bio targets.