Serological characteristics of peanut allergy in children
Serological characteristics of peanut allergy in children
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DOI：
10.1046/j.1398-9995.2003.00300.x
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发表时间：
2003-12-01
期刊：
影响因子：
12.4
通讯作者：
Scheinmann, P
中科院分区：
文献类型：
--
作者：
Bernard, H;Paty, E;Scheinmann, P
背景：食物激发试验被认为是诊断特异性食物过敏的一种很好的临床工具。然而，在花生过敏的情况下，由于反应的严重性，可能很难执行。对不同花生过敏原的特异性免疫球蛋白E（IgE）反应的定量也有助于改善诊断。根据过敏反应的严重程度对不同患者组进行全花生蛋白提取物和Ara h 1和Ara h 2的IgE反应的特征。方法：采用全蛋白提取物或纯化花生蛋白和乙酰胆碱酯酶标记的抗人IgE单克隆抗体，通过酶联免疫吸附试验对96名儿童进行特异性血清IgE分析。花生特异性IgE水平和分类之间的平行观察到五组和亚组的患者症状的严重程度增加，特别是在组内的最高严重程度。此外，最高频率的阳性反应和最高水平的特异性IgE观察与整个花生蛋白extract.Conclusion：在回顾性评价花生过敏儿童，我们已经表明，定量花生特异性IgE可用于避免食物的挑战，特别是在严重反应的情况下。当与Ara h 1和Ara h 2相比时，全花生蛋白提取物似乎是进行测试的最合适的过敏原。
Background: Food challenge is considered an excellent clinical tool for the diagnosis of specific food allergy. However in the case of peanut allergy it may be difficult to perform because of the severity of the reactions. The quantitation of a specific immunoglobulin E (IgE) response to different peanut allergens could also contribute to the improvement of the diagnosis. We characterized the IgE response to a whole peanut protein extract and to Ara h 1 and Ara h 2 in different groups of patients classified according to the severity of their allergic reactions.Methods: Specific serum IgE were analyzed in 96 children by enzyme-linked immunosorbent assay tests using a whole protein extract or purified peanut proteins and anti-human IgE monoclonal antibodies labeled with acetylcholinesterase.Results: A parallel was observed between levels of peanut-specific IgE and the classification in five groups and subgroups of patients upon increasing severity of symptoms, especially within the group of highest severity. Moreover, the highest frequency of positive response and the highest levels of specific IgE were observed with whole peanut protein extract.Conclusion: In a retrospective evaluation of peanut allergy in children, we have shown that quantitation of peanut-specific IgE could be used to avoid a food challenge particularly in the case of severe reactions. When compared to Ara h 1 and Ara h 2, whole peanut protein extract appeared to be the most appropriate allergen to perform the test.