Morphogenesis of Coronavirus HCoV-NL63 in Cell Culture: A Transmission Electron Microscopic Study.
Morphogenesis of Coronavirus HCoV-NL63 in Cell Culture: A Transmission Electron Microscopic Study.
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DOI：
10.2174/1874279300802010052
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发表时间：
2009-01
期刊：
影响因子：
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通讯作者：
J. Orenstein;Bridget S Banach;S. Baker
中科院分区：
文献类型：
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作者：
J. Orenstein;Bridget S Banach;S. Baker
NL 63（HCoV-NL 63）是最近发现的一种人类冠状病毒，可导致婴幼儿呼吸道疾病。NL 63有效地感染人气道细胞培养物的LLCMK 2细胞和纤毛上皮细胞。对NL 63感染的LLCMK 2细胞的透射电子显微镜（TEM）研究显示，病毒体呈球形，有刺突，直径范围为75至115 nm。病毒复制主要发生在粗面内质网（RER），核周和细胞质，以及高尔基体。偶尔观察到质膜出芽。随着病毒产量的增加，异常病毒形式出现的频率更高。感染细胞中存在不寻常的包涵体，包括管状和层状结构。观察到多形性双膜结合囊泡（DMV），直径约为140至210 nm。病毒通过胞吐和细胞裂解释放。总之，我们报告了通过TEM分析观察到的NL 63感染的关键形态学特征。
NL63 (HCoV-NL63) is a recently discovered human coronavirus that causes respiratory disease in infants and young children. NL63 productively infects LLCMK2 cells and ciliated epithelial cells of human airway cell cultures. Transmission electron microscopic (TEM) studies of NL63 infected LLCMK2 cells revealed that virions are spherical, spiked, and range from 75 to 115 nm in diameter. Virus replication predominantly occurs on the rough endoplasmic reticulum (RER), both perinuclear and cytoplasmic, and the Golgi. Plasma membrane budding was occasionally observed. As virus production increased, aberrant viral forms appeared with greater frequency. Unusual inclusions were present in infected cells including tubular and laminated structures. Pleomorphic double membrane-bound vesicles (DMV), measuring roughly 140 to 210 nm in diameter, were observed. The virus was released via exocytosis and cell lysis. In summary, we report the key morphologic characteristics of NL63 infection observed by TEM analysis.