Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells
Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells
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DOI：
10.1038/sj.onc.1206631
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发表时间：
2003-09-18
期刊：
影响因子：
8
通讯作者：
Gabius, HJ
中科院分区：
文献类型：
--
作者：
Kopitz, J;André, S;Gabius, HJ
半乳糖凝集素-7 （p53诱导基因1）的细胞外功能在很大程度上是未知的。在神经母细胞瘤细胞（SK-N-MC）表面，神经节苷脂苷酶活性上调导致GM(1)密度增加，是细胞从增殖向分化转变的关键因素。我们通过固相和细胞实验表明，这种神经节苷脂的糖链是半乳糖凝集素-7的配体。在血清补充的增殖试验中，半乳糖凝集素-7减少了神经母细胞瘤细胞的生长，而没有出现经典细胞凋亡的特征。半乳糖凝集素-3的存在阻断了这种作用，其机制类似于半乳糖凝集素-1。因此，尽管半乳糖凝集素-7与半乳糖凝集素-1结构不同，但由于碳水化合物的结合，它们对神经母细胞瘤的生长具有类似的控制作用。除了细胞内p53相关的促凋亡活性外，半乳糖凝集素-7，作为细胞表面的一种凝集素，似乎能够减少易感系统中的癌细胞增殖。
The extracellular functions of galectin-7 (p53-induced gene 1) are largely unknown. On the surface of neuroblastoma cells (SK-N-MC), the increased GM(1) density, a result of upregulated ganglioside sialidase activity, is a key factor for the switch from proliferation to differentiation. We show by solid-phase and cell assays that the sugar chain of this ganglioside is a ligand for galectin-7. In serum-supplemented proliferation assays, galectin-7 reduced neuroblastoma cell growth without the appearance of features characteristic for classical apoptosis. The presence of galectin-3 blocked this effect, which mechanistically resembles that of galectin-1. By virtue of carbohydrate binding, galectin-7 thus exerts neuroblastoma growth control similar to galectin-1 despite their structural differences. In addition to p53-linked proapoptotic activity intracellularly, galectin-7, acting as a lectin on the cell surface, appears to be capable of reducing cancer cell proliferation in susceptible systems.