Phylogenetic profile of gut microbiota in healthy adults after moderate intake of red wine
Phylogenetic profile of gut microbiota in healthy adults after moderate intake of red wine
复制标题
DOI：
10.1002/mnfr.201600620
复制
发表时间：
2017-03-01
影响因子：
5.2
通讯作者：
Requena, Teresa
中科院分区：
文献类型：
--
作者：
Barroso, Elvira;Munoz-Gonzalez, Irene;Requena, Teresa
范围：人们对如何通过饮食习惯来改变人类的结肠菌群越来越感兴趣。我们研究了适量摄入红酒对15名健康志愿者结肠微生物群的影响，这与这种饮料中存在的高浓度多酚有关。根据志愿者对多酚的代谢能力，他们被分为高、中等和低多酚代谢（代谢型），并将结果与5名对照组（不饮酒）受试者的结果进行比较。方法和结果：我们分析了饮用葡萄酒前后1个月粪便微生物群的组成、多样性和动态。16S rDNA测序共检测到2324个种型，其中仅有30个种型出现在平均相对频率的0.5%以上，占总分类分配的84.6%。与葡萄酒摄入或代谢类型相比，个体对样本的聚集性更强，然而，在葡萄酒摄入后，观察到多样性的增加。结论：本研究结果表明，全球粪便微生物多样性的增加与饮用红葡萄酒有关，证实了不同个体微生物群的高度变异性，并表明其单一微生物群组成对小而短期的饮食变化具有稳定性。
Scope: There is growing interest in understanding how human colonicmicrobiota can be modified by dietary habits. We examined the influence of moderate red wine intake on the colonic microbiota of 15 healthy volunteers, related to the high concentration of polyphenols present in this beverage. The volunteers were classified into high, moderate, and low polyphenol metabolizers (metabotypes) due to their ability to metabolize polyphenols and the results were compared with that of five control (no wine intake) subjects.Methods and results: We analyzed the composition, diversity, and dynamics of their fecal microbiota before and after 1 month of wine consumption. The 16S rDNA sequencing allowed detection of 2324 phylotypes, of which only 30 were found over the 0.5% of mean relative frequency, representing 84.6% of the total taxonomical assignments. The samples clustered more strongly by individuals than by wine intake or metabotypes, however an increase in diversity, after the wine intake, was observed.Conclusion: The results of this study suggest an increase in the global fecal microbial diversity associated to the consumption of red wine, confirm the high variability of the microbiota from different individuals, and show the stability of their singular microbiota composition to small and short-term dietary changes.