SELECTIVE MODULATION OF DESENSITIZATION AT AMPA VERSUS KAINATE RECEPTORS BY CYCLOTHIAZIDE AND CONCANAVALIN-A
SELECTIVE MODULATION OF DESENSITIZATION AT AMPA VERSUS KAINATE RECEPTORS BY CYCLOTHIAZIDE AND CONCANAVALIN-A
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DOI：
10.1016/0896-6273(93)90220-l
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发表时间：
1993-12-01
期刊：
影响因子：
16.2
通讯作者：
BUONANNO, A
中科院分区：
文献类型：
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作者：
PARTIN, KM;PATNEAU, DK;BUONANNO, A
环噻嗪增强非洲爪蟾卵母细胞中重组谷氨酸受体的反应表现出绝对的选择性AMPA与红藻氨酸受体。与此相反，伴刀豆球蛋白A强烈增强红藻氨酸，但不是AMPA受体的反应。转染AMPA受体的HEK 293细胞的快速脱敏被环噻嗪阻断，但仅被伴刀豆球蛋白A弱减弱。在红藻氨酸受体的脱敏被刀豆球蛋白A阻断，但不受环噻嗪。这些调节剂的选择性作用后，从不同的家庭亚基共表达建议独立的功能AMPA和红藻氨酸受体的组装。背根神经节mRNA的北方毕奥分析揭示了GluR5的主要表达，表明在感觉神经元中由伴刀豆球蛋白A而不是环噻嗪对脱敏的调节准确地预测了天然谷氨酸受体的亚基表达。
Potentiation by cyclothiazide of recombinant glutamate receptor responses in Xenopus oocytes showed absolute selectivity for AMPA versus kainate receptors. In contrast, concanavalin A strongly potentiated responses at kainate but not AMPA receptors. Rapid desensitization in HEK 293 cells transfected with AMPA receptors was blocked by cyclothiazide, but only weakly attenuated by concanavalin A. Desensitization at kainate receptors was blocked by concanavalin A but unaffected by cyclothiazide. Selective effects of these modulators following coexpression of subunits from different families suggest independent assembly of functional AMPA and kainate receptors. Northern biot analysis of mRNA for dorsal root ganglia revealed a predominant expression of GluR5, indicating that modulation of desensitization by concanavalin A but not cyclothiazide in sensory neurons accurately predicts subunit expression for native glutamate receptors.