Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)
Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)
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稻瘿矮化病毒对其媒介昆虫Recilia dorsalis（半翅目：叶蝉科）的不利影响
DOI：
10.1094/pdis-06-15-0713-re
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发表时间：
2016-04-01
期刊：
影响因子：
4.5
通讯作者：
Wei, Taiyun
中科院分区：
文献类型：
--
作者：
Chen, Yong;Lu, Chengcong;Wei, Taiyun
水稻瘤矮病毒（Rice gall dwarf virus，RGDV）是一种通过叶蝉介体Recilia dorsalis以持续繁殖方式传播的植物呼肠孤病毒，严重威胁东南亚和中国南方的水稻生产。评价RGDV对红腹锦鸡儿的直接影响。dorsalis，采用滤纸水浸泡培养法，从带毒雌虫产卵中建立了一个带毒叶蝉种群。生活史参数表明，与不含病毒的对照种群相比，该病毒在所有生物指标方面对其媒介有害，包括成虫的存活率、羽化率、繁殖力和寿命降低。这些发现得到了系统监测R.不同越冬代的dorsalis。为了更好地阐明RGDV对其载体的不利影响，我们测量了生殖力在分子水平上使用定量逆转录聚合酶链反应和蛋白质印迹分析，这揭示了差异表达的卵黄蛋白原（Vg）在viruliferous与nonviruliferous成年女性。我们推断RGDV降低了Vg转录物和蛋白质产物的水平，导致其载体的繁殖力较低。总之，这项研究表明RGDV如何对R产生不利影响。dorsalis，这阻碍了昆虫的含病毒种群的扩张。
Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis in a persistent-propagative manner. To assess the direct effects of RGDV on R. dorsalis, we established an infected leafhopper population from eggs laid by viruliferous females using the water-soaked filter paper culture method. Life history parameters indicated that the virus was harmful to its vector in terms of all biotic indices, including reduced survival rate, emergence rate, fecundity, and longevity of adults, compared with a nonviruliferous control population. Those findings were supported by systematic monitoring of viruliferous rates of R. dorsalis in different overwintering generations. To better elucidate the adverse effects of RGDV on its vector, we measured fecundity at the molecular level using quantitative reverse transcription polymerase chain reaction and Western blot assays, which revealed differential expression of vitellogenin (Vg) in viruliferous versus nonviruliferous adult females. We infer that RGDV reduced levels of Vg transcript and protein product, resulting in the lower fecundity of its vector. Overall, this study demonstrates how RGDV exerts an adverse effect on R. dorsalis, which hinders the expansion of viruliferous populations of the insect.