Immune response to a thymus-dependent form of B512 dextran requires the presence of Lyb-5+ lymphocytes.
Immune response to a thymus-dependent form of B512 dextran requires the presence of Lyb-5+ lymphocytes.
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DOI：
10.1084/jem.157.2.657
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发表时间：
1983-02-01
期刊：
影响因子：
--
通讯作者：
Paul WE
中科院分区：
文献类型：
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作者：
Stein KE;Zopf DA;Miller CB;Johnson BM;Mongini PK;Ahmed A;Paul WE
对B512葡聚糖（Dex）的免疫应答的个体发生的研究表明，直到12周龄才达到与成年小鼠相等的抗体应答。我们已经检查了用胸腺依赖性抗原异麦芽糖己糖基-钥孔血蓝蛋白（IM 6-KLH）免疫后的抗Dex应答，并表明交叉反应性抗Dex应答的发展与LyB-5+ B细胞的发展平行。在用IM 6-KLH免疫后，在3至12周龄之间的小鼠中达到成人水平的抗Dex抗体，此时Lyb-5+细胞已经达到成人水平。新生小鼠，在出生后1天或1周免疫，未能产生显着量的抗Dex抗体，虽然他们与IM 6-KLH免疫后产生IM 6特异性抗体。数据支持来自这些实验的结论，即即使当免疫抗原是胸腺依赖性的时，Lyb-5+细胞也是抗多糖应答所必需的，包括IM 6-KLH不能刺激具有xid缺陷的小鼠中的正常抗Dex应答，以及在正常成年小鼠中的直接证明，从用IM 6-KLH致敏的小鼠的脾中消除Lyb-5+细胞，KLH消除了这些细胞转移抗Dex反应的能力。这些数据表明，成年动物中表达的B细胞库是偏斜的，使得绝大多数能够对多糖决定簇应答的B细胞处于LyB-5+亚群中。
Studies of the ontogeny of the immune response to B512 dextran (Dex) show that antibody responses equal to those of adult mice are not attained until 12 wk of age. We have examined the anti-Dex response after immunization with a thymus-dependent antigen isomaltohexaosyl- keyhole limpet hemocyanin (IM6-KLH) and have shown that the development of the cross-reacting anti-Dex response parallels the development of Lyb-5+ B cells. Adult levels of anti-Dex antibody after immunization with IM6-KLH are achieved in mice between 3 and 12 wk of age, a time when Lyb-5+ cells have reached adult levels. Neonatal mice, immunized at 1 d or 1 wk after birth, failed to produce a significant amount of anti-Dex antibodies, although they did produce IM6-specific antibodies after immunization with IM6-KLH. Data, which support the conclusion from these experiments that Lyb-5+ cells are required for an anti- polysaccharide response even when the immunizing antigen is thymus- dependent, include the failure of IM6-KLH to stimulate a normal anti- Dex response in mice with the xid defect and the direct demonstration in normal adult mice that elimination of Lyb-5+ cells from spleens of mice primed with IM6-KLH abolishes the ability of these cells to transfer an anti-Dex response. The data imply that the expressed B cell repertoire in adult animals is skewed such that the vast majority of B cells capable of responding to polysaccharide determinants are in the Lyb-5+ subset.