Controlled cell adhesion on PEG-based switchable surfaces
Controlled cell adhesion on PEG-based switchable surfaces
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DOI：
10.1002/anie.200801202
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发表时间：
2008-01-01
影响因子：
16.6
通讯作者：
Lutz, Jean-Francois
中科院分区：
文献类型：
--
作者：
Wischerhoff, Erik;Uhlig, Katja;Lutz, Jean-Francois
化学品。甲基丙烯酸2-(2-甲氧基乙氧基)乙酯(Aldrich，95%)、低聚(乙二醇甲基醚)甲基丙烯酸酯(Aldrich，Mn=475g·m ol-1)、甲基丙烯酸羟乙酯(Aldrich，98%)、溴化铜(Aldrich，99%)、L抗坏血酸(Aldrich，99%)、2，2‘-联吡啶(Aldrich，98%)、3-巯基-1-丙磺酸钠(Aldrich 90%)、磷酸盐缓冲盐水(PBS，生化片、Fluka)、聚苯磺酸盐(Aldrich，MW=70000)和聚烯丙胺盐酸盐(Aldrich，Aldrich，98%)MW=70000)，按接收时使用。按已发表的方法合成了二硫化物ATRP引发剂1。[1]溴化铜(Acros，95%)用冰醋酸洗涤，以去除任何可溶的氧化物种，过滤，乙醇洗涤，干燥。在使用前，所有表面都要浸泡在0.5g高锰酸钾溶液中，浸泡在100毫升浓硫酸中1小时。
Chemicals. 2-(2-Methoxyethoxy) ethyl methacrylate (Aldrich, 95%), oligo (ethylene glycol) methyl ether methacrylate (Aldrich, Mn= 475 g· mol-1), 2-hydroxyethyl methacrylate (Aldrich, 98%), copper (II) bromide (Aldrich, 99%), L-ascorbic acid (Aldrich, 99%), 2, 2’bipyridyl (Fluka, 98%), sodium 3-mercapto-1-propanesulfonate (Aldrich 90%), phosphate buffered saline (PBS, BioChemika tablets, Fluka), poly (styrene sulfonate)(Aldrich, Mw= 70000), and poly (allylamine hydrochloride)(Aldrich, Mw= 70000) were used as received. The disulfide ATRP initiator 1 was synthesized according to published procedures.[1] Copper (I) bromide (Acros, 95%) was washed with glacial acetic acid in order to remove any soluble oxidized species, filtered, washed with ethanol and dried. Prior to use, all surfaces were cleaned by immersion in a solution of 0.5 g of potassium permanganate in 100 ml of concentrated H2SO4 for 1 h.