Nanoparticles Containing Curcumin Useful for Suppressing Macrophages In Vivo in Mice.
Nanoparticles Containing Curcumin Useful for Suppressing Macrophages In Vivo in Mice.
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DOI：
10.1371/journal.pone.0137207
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hinuma S
中科院分区：
文献类型：
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作者：
Amano C;Minematsu H;Fujita K;Iwashita S;Adachi M;Igarashi K;Hinuma S
为了探索脂质体抑制巨噬细胞的新方法，我们通过细胞培养试验筛选食物成分。姜黄素是对巨噬细胞具有抑制作用的最强化合物之一。随后，我们尝试了各种方法来制备脂质体姜黄素，并最终成功地制备脂质体与足够量的姜黄素抑制巨噬细胞通过掺入姜黄素和牛血清白蛋白的复合物。所得到的纳米颗粒，含有姜黄素的脂质体的直径范围为60至100 nm。流式细胞术分析显示，在腹腔内给予小鼠含有姜黄素的脂质体后，这些脂质体主要被F4/80、CD 36和CD 11b抗原阳性的巨噬细胞掺入。小鼠腹膜细胞在体内注射含姜黄素的脂质体后，其产生白细胞介素-6的活性明显降低。给予含姜黄素的脂质体后，未观察到小鼠体重和存活率的重大变化。这些结果表明，含有姜黄素的脂质体是安全的，并可用于选择性抑制小鼠体内巨噬细胞。
To explore a novel method using liposomes to suppress macrophages, we screened food constituents through cell culture assays. Curcumin was one of the strongest compounds exhibiting suppressive effects on macrophages. We subsequently tried various methods to prepare liposomal curcumin, and eventually succeeded in preparing liposomes with sufficient amounts of curcumin to suppress macrophages by incorporating a complex of curcumin and bovine serum albumin. The diameter of the resultant nanoparticles, the liposomes containing curcumin, ranged from 60 to 100 nm. Flow cytometric analyses revealed that after intraperitoneal administration of the liposomes containing curcumin into mice, these were incorporated mainly by macrophages positive for F4/80, CD36, and CD11b antigens. Peritoneal cells prepared from mice injected in vivo with the liposomes containing curcumin apparently decreased interleukin-6-producing activities. Major changes in body weight and survival rates in the mice were not observed after administrating the liposomes containing curcumin. These results indicate that the liposomes containing curcumin are safe and useful for the selective suppression of macrophages in vivo in mice.