DROSOPHILA DNA TOPOISOMERASE-I IS ASSOCIATED WITH TRANSCRIPTIONALLY ACTIVE REGIONS OF THE GENOME
DROSOPHILA DNA TOPOISOMERASE-I IS ASSOCIATED WITH TRANSCRIPTIONALLY ACTIVE REGIONS OF THE GENOME
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DOI：
10.1073/pnas.81.22.6958
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
ELGIN, SCR
中科院分区：
文献类型：
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作者：
FLEISCHMANN, G;PFLUGFELDER, G;ELGIN, SCR
用亲和纯化的抗体进行免疫荧光染色，观察DNA拓扑异构酶I在果蝇多线染色体上的分布。该酶优先与活性位点相关，如突出的喷烟染色所示。热休克基因座87 A-87 C在热休克诱导后染色，但在热休克诱导前不染色。拓扑异构酶I的分布与RNA聚合酶II的分布的详细比较揭示了类似的，但不相同的关联模式。拓扑异构酶I也被发现与核仁（RNA聚合酶I的转录位点）相关。
The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomerase I with that of RNA polymerase II reveals a similar, although not identical, pattern of association. Topoisomerase I is also found in association with the nucleolus, the site of transcription by RNA polymerase I.