Enhancement of native and phosphorylated TDP-43 immunoreactivity by proteinase K treatment following autoclave heating
Enhancement of native and phosphorylated TDP-43 immunoreactivity by proteinase K treatment following autoclave heating
复制标题
高压灭菌后通过蛋白酶 K 处理增强天然和磷酸化 TDP-43 免疫反应性
DOI：
10.1111/j.1440-1789.2010.01184.x
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发表时间：
2011
期刊：
影响因子：
2.3
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Mori F;et al.
TDP-43是肌萎缩侧索硬化症（ALS）和额颞叶变性伴TDP-43（FTLD-TDP）的主要疾病蛋白。为了评价蛋白酶K（PK）治疗在天然和磷酸化TDP-43蛋白抗原修复中的有效性，我们检查了散发性ALS和FTLD-TDP患者以及对照受试者的颞叶皮质和脊髓。热修复后的PK处理增强了对照中天然TDP-43以及ALS和FTLD-TDP中天然和磷酸化TDP-43的免疫反应性。在病变中发现大量TDP-43阳性神经纤维丝，其中常规免疫组织化学显示主要包涵体为细胞质。这种修复方法是证明TDP-43病理学的最佳免疫组织化学技术，尤其是在神经胶质瘤中。
TDP‐43 is a major disease protein in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with TDP‐43 (FTLD‐TDP). To evaluate the effectiveness of proteinase K (PK) treatment in antigen retrieval for native and phosphorylated TDP‐43 protein, we examined the temporal cortex and spinal cord from patients with sporadic ALS and FTLD‐TDP and control subjects. PK treatment following heat retrieval enhanced the immunoreactivity for native TDP‐43 in controls as well as for native and phosphorylated TDP‐43 in ALS and FTLD‐TDP. A significant number of TDP‐43‐positive neuropil threads were demonstrated in lesions, in which routine immunohistochemistry revealed that the predominant inclusions are cytoplasmic. This retrieval method is the best of immunohistochemical techniques for demonstrating TDP‐43 pathology, especially in the neuropil.