Differential messenger RNA expression of complexins in mouse brain
Differential messenger RNA expression of complexins in mouse brain
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DOI：
10.1016/j.brainresbull.2003.12.003
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发表时间：
2004-03-01
影响因子：
3.8
通讯作者：
Morton, AJ
中科院分区：
文献类型：
--
作者：
Freeman, W;Morton, AJ
复合体蛋白(CPLXs)是一种小分子异构体蛋白，能与可溶性的NSF附着蛋白受体(SNARE)结合，调节神经递质的释放。大脑中存在CPLX的两种亚型，CPLXI和CPLXII。它们在大脑皮层和小脑中有不同的分布，其中CPLXI见于轴体末梢，CPLXII见于轴突末梢。由于在大脑皮层和小脑，轴突终末是抑制性的，而轴突终末是兴奋性的，因此，人们认为CPLXI调节抑制性，CPLXII调节兴奋性递质的释放。本实验采用原位杂交的方法，研究了CPLXI和CPLXII在小鼠脑内的mRNA分布。我们发现，虽然CPLX在不同的细胞群中表达，但它们既不与特定的神经递质分离，也不与不同类别的递质作用分离。例如，虽然CPLXII是大脑皮层输出神经元(谷氨酸能兴奋)的主要亚型，但它也是纹状体中等刺状(GABA能抑制)神经元的主要亚型。我们认为CPLX的功能作用不仅取决于神经递质的特性，还取决于连接表达CPLX的神经元的电路。因此，CPLXII在基底节和皮质神经元中的主要表达表明，CPLXII在认知、情绪行为和随意运动控制中发挥作用，而CPLXII的表达模式表明，CPLXII在运动学习程序和感觉加工中起主要作用。(C)2004 Elsevier Inc.保留所有权利。
Complexins (CPLXs) are small isomeric proteins that bind to the soluble NSF-attachment protein receptor (SNARE) complex and modulate neurotransmitter release. Two isoforms of CPLX exist in the brain, CPLXI and CPLXII. These are differentially distributed in the cortex and cerebellum, with CPLXI found in axosomatic terminals and CPLXII in axodendritic terminals. Since in cortex and cerebellum axosomatic terminals are inhibitory and axodendritic terminals are excitatory, it has been assumed that CPLXI modulates inhibitory and CPLXII modulates excitatory transmitter release. Here we used in situ hybridisation to study the mRNA distribution of CPLXI and CPLXII in mouse brain. We show that while CPLXs are expressed in distinct cell populations, they do not segregate with either particular neurotransmitters, or different classes of transmitter action. For example, while CPLXII is the dominant isoform in the output (glutamatergic excitatory) neurons of the cortex, it is also the dominant isoform in medium spiny (GABAergic inhibitory) neurons of the striatum. We suggest that the functional role of CPLXs depends not only on the identity of the neurotransmitter, but also upon the circuitry connecting the neurons in which they are expressed. Thus, the predominant expression of CPLXII in neurons of the basal ganglia and cortex suggests a role in cognition, emotional behaviour and control of voluntary movement, while the pattern of CPLXI expression suggests a primary role in motor learning programs and sensory processing. (C) 2004 Elsevier Inc. All rights reserved.