Effect of pre-incubation temperature on susceptibility of Galleria mellonella larvae to infection by Candida albicans
Effect of pre-incubation temperature on susceptibility of Galleria mellonella larvae to infection by Candida albicans
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DOI：
10.1007/s11046-007-9069-9
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发表时间：
2008-01-01
期刊：
影响因子：
5.5
通讯作者：
Kavanagh, Kevin
中科院分区：
文献类型：
--
作者：
Mowlds, Peter;Kavanagh, Kevin
越来越多地使用昆虫来评估微生物病原体的毒力和确定抗菌药物的疗效。在4℃或37℃孵育24 h后，与30℃预孵育24 h的幼虫相比，在4℃或37℃孵育24 h后，大蜡蛾的幼虫对白色念珠菌的侵染表现出更强的抵抗力。4℃或37℃孵育导致血细胞密度增加，编码加雷米星、转铁蛋白、可诱导金属蛋白酶抑制物(IMPI)和加洛米星的基因表达增加。这些基因的表达高峰记录在4℃或37℃孵化开始后的大约24小时。这些结果表明，幼虫暴露在温和的热休克条件下，通过增加血细胞数量和增加抗菌肽的表达来诱导保护性细胞和体液免疫反应。
The use of insects for evaluating the virulence of microbial pathogens and for determining the efficacy of antimicrobial drugs is increasing. When larvae of the greater wax moth Galleria mellonella were incubated at 4 or 37 degrees C for 24 h. prior to infection, they manifested increased resistance to infection by the yeast Candida albicans compared to larvae that had been pre-incubated for 24 h at 30 degrees C. Incubation at 4 or 37 degrees C led to an increase in haemocyte density and the expression of genes coding for gallerimycin, transferrin, an inducible metalloproteinase inhibitor (IMPI) and galiomicin. Peak expression of these genes was recorded at approximately 24 h after the commencement of the 4 or 37 degrees C incubation. These results indicate that exposure of larvae to mild thermal shock conditions induces a protective cellular and humoral immune response mediated by increased numbers of haemocytes and elevated expression of antimicrobial peptides.