Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion
Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion
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DOI：
10.1097/iae.0b013e31815ec32d
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发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Agardh, Per Hagert Elisabet
中科院分区：
文献类型：
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作者：
Gustavsson, Carin;Agardh, Carl-David;Agardh, Per Hagert Elisabet
目的：观察欧米伽-3-多不饱和脂肪酸(DHA)或3-羟基-3-甲基戊二酰辅酶A还原酶抑制剂(普伐他汀)对非糖尿病和糖尿病大鼠视网膜缺血再灌注损伤的炎症反应。方法：采用链佐菌素诱导糖尿病模型，结扎视神经和血管诱导视网膜缺血，再灌注1小时或24小时。手术前5分钟，静脉注射DHA、普伐他汀或赋形剂(乙醇)。比较缺血组与非缺血组、糖尿病非缺血组与非缺血组肿瘤坏死因子-α、白介素6、半胱氨酸天冬氨酸氨基转移酶-1、白介素11-β、P-选择素、血管细胞黏附分子-1、细胞间黏附分子-1的表达。
Purpose: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin).Methods: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-11 beta, P-selectin, vascular cellular adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 were compared between ischemic and nonischemic retinas as well as diabetic and nondiabetic nonischemic retinas.Results: Ischemia induced increased expressions of TNF-alpha (P