Bilateral microinjections of vigabatrin in the central piriform cortex retard AMYGDALA kindling in rats
Bilateral microinjections of vigabatrin in the central piriform cortex retard AMYGDALA kindling in rats
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DOI：
10.1016/j.neuroscience.2004.08.013
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发表时间：
2004-12
期刊：
影响因子：
3.3
通讯作者：
Kerstin Schwabe;Ulrich Ebert;Wolfgang Löscher
中科院分区：
文献类型：
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作者：
Kerstin Schwabe;Ulrich Ebert;Wolfgang Löscher
梨状皮质（PC）是哺乳动物嗅觉皮质中最大的区域，与边缘系统结构（包括杏仁核、海马和内嗅皮质）有很强的联系。先前在啮齿动物中的各种研究表明，PC可能在边缘点燃的发展和维持中非常重要，即广泛使用的颞叶癫痫模型。前PC和后PC之间的过渡区（此处称为中央PC）中的GABA能抑制似乎特别参与导致点燃癫痫发作进展的过程。这促使我们研究是否GABA水平的升高，在这个分区的PC双边微量注射氨己烯酸能够抑制杏仁核点燃。每天刺激大鼠一次，直到完全点燃（第5阶段）癫痫发作。在第一次刺激前24 h以及第5次和第10次刺激前6 h注射氨己烯酸（10 μg），与对照组相比，点燃发展所需的刺激次数约为对照组的两倍。这种明显的延迟点燃收购主要是由于一个显着的抑制从阶段1到阶段2和阶段3到阶段4癫痫发作的进展，表明微量注射氨己烯酸到中央PC显着抑制的进展和继发性泛化的局灶性癫痫发作从杏仁核。
The piriform cortex (PC) is the largest region of the mammalian olfactory cortex with strong connections to limbic structures, including the amygdala, hippocampus, and entorhinal cortex. Various previous studies in rodents suggest that the PC might be very important in the development and maintenance of limbic kindling, i.e. a widely used model of temporal lobe epilepsy. GABAergic inhibition in the transition zone between the anterior and posterior PC, termed here central PC, seems to be particularly involved in the processes leading to progression of kindled seizures. This prompted us to study whether elevation of GABA levels in this subregion of the PC by bilateral microinjection of vigabatrin is capable of suppressing amygdala kindling. Rats were stimulated once daily until fully kindled (stage 5) seizures had developed. Vigabatrin (10 μg) was injected 24 h before the first stimulation as well as 6 h before the 5th and 10th stimulation, which approximately doubled the number of stimulations required for kindling development compared with controls. This marked retardation of kindling acquisition was predominantly due to a significant inhibition of the progression from stage 1 to stage 2 and stage 3 to stage 4 seizures, demonstrating that microinjection of vigabatrin into the central PC markedly inhibits the progression and secondary generalization of focal seizures emanating from the amygdala.