Alveolar hyperoxic injury in rabbits receiving exogenous surfactant.
Alveolar hyperoxic injury in rabbits receiving exogenous surfactant.
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接受外源性表面活性剂的兔子的肺泡高氧损伤。
DOI：
10.1152/jappl.1989.66.3.1087
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Matalon,S
中科院分区：
文献类型：
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作者：
Loewen,GM;Holm,BA;Milanowski,L;Wild,LM;Notter,RH;Matalon,S
我们之前已经证明，在暴露于100% O2 64小时后，在家兔中灌注小牛肺表面活性剂提取物（CLSE），可减轻返回室内空气后肺部病理的进展。物理学报。62:756-761,1987)。在本研究中，我们研究了是否可以通过在高氧暴露期间连续注入CLSE来预防或减少高氧肺损伤的发生和发展。兔暴露于100% O2。将CLSE （125 mg，约170 μ mol磷脂）悬浮在10 ml无菌生理盐水中，并于24 h O2中开始，无生理或生化损伤检测时，每隔24 h气管内灌注肺内。对照组兔子呼吸100%的氧气，并接受等量的生理盐水或根本不注射。与生理盐水对照组相比，clse灌注家兔在整个暴露期间动脉PO2 （Pao2）值较高，存活时间更长[120 +/- 4比102 +/- 4 (SE) h；N大于或等于10；P < 0.05]。在缺氧72小时，clse灌注家兔的肺泡可灌洗磷脂水平（12.5 +/- 1.5 vs. 5 +/- 1 mumol/kg）和总肺活量（41 +/- 2 vs. 25 +/- 3.5 ml/kg）显著升高，肺泡蛋白水平（24 +/- 3 vs. 52 +/- 8 mg/kg）、最小表面张力（2 +/- 1 vs. 26.1 dyn/cm）和肺干重（5.9 +/- 0.2 vs. 6.5 +/- 0.3）显著降低。在缺氧72小时后，CLSE和盐水灌注家兔的肺均显示弥漫性高氧损伤。然而，前者的肺不张不明显。我们得出结论，CLSE的灌注限制了家兔肺泡上皮高氧肺损伤的发生和发展。
We have previously demonstrated that instillation of a calf lung surfactant extract (CLSE) in rabbits after exposure to 100% O2 for 64 h mitigates the progression of lung pathology after return to room air (J. Appl. Physiol. 62: 756–761, 1987). In the present study, we investigated whether we could prevent or reduce the onset and development of hyperoxic lung injury by sequential instillations of CLSE during the hyperoxic exposure. Rabbits were exposed to 100% O2. CLSE (125 mg, approximately 170 mumol of phospholipid) was suspended in 10 ml of sterile saline and instilled intratracheally into their lungs, starting at 24 h in O2, a time at which no physiological or biochemical injury was detected, and at 24-h intervals thereafter. Control rabbits breathed 100% O2 and received either equal volumes of saline or no instillations at all. CLSE-instilled rabbits had higher arterial PO2 (Pao2) values throughout the exposure period and survived longer when compared with saline controls [120 +/- 4 vs. 102 +/- 4 (SE) h; n greater than or equal to 10; P less than 0.05]. At 72 h in O2, CLSE-instilled rabbits had significantly higher lavageable alveolar phospholipid levels (12.5 +/- 1.5 vs. 5 +/- 1 mumol/kg) and total lung capacities (41 +/- 2 vs. 25 +/- 3.5 ml/kg) and lower levels of alveolar protein (24 +/- 3 vs. 52 +/- 8 mg/kg), minimum surface tension (2 +/- 1 vs. 26.1 dyn/cm), and lung wet-to-dry weights (5.9 +/- 0.2 vs. 6.5 +/- 0.3). After 72 h in O2, lungs from both CLSE- and saline-instilled rabbits showed evidence of diffuse hyperoxic injury. However, atelectasis was less prominent in the former. We concluded that instillation of CLSE limits the onset and development of hyperoxic lung injury to the alveolar epithelium of rabbits.