AN NAD DERIVATIVE PRODUCED DURING TRANSFER-RNA SPLICING - ADP-RIBOSE 1''-2'' CYCLIC PHOSPHATE
AN NAD DERIVATIVE PRODUCED DURING TRANSFER-RNA SPLICING - ADP-RIBOSE 1''-2'' CYCLIC PHOSPHATE
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DOI：
10.1126/science.8392224
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发表时间：
1993-07-09
期刊：
影响因子：
56.9
通讯作者：
PHIZICKY, EM
中科院分区：
文献类型：
--
作者：
CULVER, GM;MCCRAITH, SM;PHIZICKY, EM
转移RNA（tRNA）剪接在酿酒酵母和人类中都是必不可少的，并且它的许多特征在两者中是相同的。在酵母中，该过程的最后一步是在连接过程中去除剪接点处产生的2'磷酸。烟酰胺腺嘌呤二核苷酸（NAD）依赖性磷酸转移酶催化2'磷酸的去除并产生小分子。这里显示，这种小分子是NAD衍生物：腺苷二磷酸（ADP）-核糖1“-2”环磷酸。证据也表明，这种分子产生于非洲爪蟾卵母细胞作为连接的tRNA的去磷酸化的结果。
Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2' phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleotide (NAD)-dependent phosphotransferase catalyzes removal of the 2' phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP)-ribose 1''-2'' cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.