Effects of 4-amino-2-trifluoromethyl-phenyl Retinate on Differentiation of Human Digestive System Tumor Cells in vitro
Effects of 4-amino-2-trifluoromethyl-phenyl Retinate on Differentiation of Human Digestive System Tumor Cells in vitro
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DOI：
10.3971/j.issn.1000-8578.2011.12.009
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发表时间：
2011-12
影响因子：
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通讯作者：
Hong Fan-qing;Chen Fei-hu;Wu Fei;Chen Hui-hui
中科院分区：
文献类型：
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作者：
Hong Fan-qing;Chen Fei-hu;Wu Fei;Chen Hui-hui
目的研究4-氨基-2-三氟甲基-苯基维甲酸酯（ATPR）对人胃癌细胞SGC-7901、人肝癌细胞Bel-7402和人结肠癌细胞HT-29增殖和分化的影响。方法体外培养实体瘤细胞，用不同浓度的ATPR处理，MTT法检测细胞增殖，ALP，结果ATPR对实体瘤细胞的生长有明显的抑制作用，且呈剂量依赖性，SGC-7901对ALP、LDH活性有抑制作用，AFP、CEA、ALP活性无抑制作用，SGC-7901对AFP、CEA、ALP活性无抑制作用，SGC-7901对ALP、LDH活性无抑制作用，SGC-7901对AFP、CEA、ALP活性无抑制作用，SGC-7901对ALP、LDH活性无抑制作用。随着ATPR浓度的升高，Bel-7402细胞LDH、γ-GT降低，HT-29细胞CEA表达增加; G 0/G1期细胞明显增多，S期细胞明显减少，细胞周期阻滞于G1期。
Objective To study the effect of 4-amino-2-trifluoromethyl-phenyl retinate(ATPR) on the proliferation and differentiation of human gastric cancer cells SGC-7901,human liver cancer cells Bel-7402 and human colon carcinoma cells HT-29.Methods Solid tumor cells were cultured in vitro and treated with ATPR of different concentration.The proliferation was detected with MTT test.ALP,LDH and γ-GT were measured by spectrophotometer for enzyme activity,AFP and CEA were measured by ELISA.The cell cycle was analyzed by flow cytometry.Results The growth of solid tumor cells treated with ATPR was inhibited in a does-dependent manner.ALP and LDH activity of SGC-7901 suppressant.AFP,LDH and γ-GT of Bel-7402 decreased while the expression of CEA of HT-29 increased.G0/G1-phase cells significantly increased while S-phase cells decreased with the elevation of ATPR concentration.Cell cycle progression was blocked in the G1 phase.Conclusion ATPR could induce differentiation of solid tumor cells.