Protein derivatization and sequential ion/ion reactions to enhance sequence coverage produced by electron transfer dissociation mass spectrometry
Protein derivatization and sequential ion/ion reactions to enhance sequence coverage produced by electron transfer dissociation mass spectrometry
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DOI：
10.1016/j.ijms.2014.06.023
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发表时间：
2015-02-01
影响因子：
1.8
通讯作者：
Hunt, Donald F.
中科院分区：
文献类型：
--
作者：
Anderson, Lissa C.;English, A. Michelle;Hunt, Donald F.
之前，我们描述了用于Orbitrap仪器的前端ETD（电子转移解离）源的实现[1]。该源有助于在质量分析之前用来自完整蛋白质的ETD的产物离子多次填充C阱。结果是所观察到的离子电流的显著增强，而不需要对来自多个质量测量的数据进行耗时的平均。在这里，我们表明，离子-离子质子转移（IIPT）反应可以用来简化ETD光谱和分散碎片离子在整个质量范围内以受控的方式。我们还表明，蛋白质衍生化可以选择性地增强在蛋白质的N-和C-末端观察到的序列信息。(C)2014爱思唯尔有限公司版权所有。
Previously, we described implementation of a front-end ETD (electron transfer dissociation) source for an Orbitrap instrument [1]. This source facilitates multiple fills of the C-trap with product ions from ETD of intact proteins prior to mass analysis. The result is a dramatic enhancement of the observed ion current without the need for time consuming averaging of data from multiple mass measurements. Here we show that ion-ion proton transfer (IIPT) reactions can be used to simplify ETD spectra and to disperse fragment ions over the entire mass range in a controlled manner. We also show that protein derivatization can be employed to selectively enhance the sequence information observed at the N- and C-termini of a protein. (C) 2014 Elsevier B.V. All rights reserved.