Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint
Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint
复制标题
DOI：
10.1111/1750-3841.15095
复制
发表时间：
2020-04-01
影响因子：
3.9
通讯作者：
Li, Dengwu
中科院分区：
文献类型：
--
作者：
Yu, Li;Wu, Wenli;Li, Dengwu
通过酚类化合物、生物活性、HPLC指纹图谱结合化学计量学分析对7种花椒皮的质量进行评价，并筛选出关键化学成分。结果表明，S2（奉贤大红袍）的总酚和芦丁含量最高，抗氧化活性最强，其次是S6（韩城狮子头）。从HPLC指纹图谱中筛选出8个共同特征峰，有效地控制了黄皮药材的质量，总贡献率为92.775%。各样本的相似性值在0.696 ~ 0.970之间。此外，金丝桃苷是主成分分析法评价金丝桃果皮质量的另一关键化学指标。据此，将7个品种划分为4组，其中4组（奉贤大红袍，S2）为优秀品种，其次是1组（S5、S6、S7）和3组（S3、S4），最后是2组（河豚晚熟，S1）。这些结果将有助于建立有效、全面的白杨种质资源评价体系。本研究不仅为兔皮质量评价提供了另一项指标，也有望为兔皮优质种质培育和食品添加剂、药品、保健品等方面的开发提供理论依据。
This work aims to evaluate the quality of seven varieties of Zanthoxylum bungeanum peels based on phenolic compounds, bioactivity, and HPLC fingerprint combined with chemometrics analysis, and pick out the key chemical compounds. As a result, S2 (Fengxian Dahongpao) showed the highest content of total phenolic and rutin, as well as the strongest antioxidant activity, followed by S6 (Hancheng Shizitou). From HPLC fingerprint, eight common characteristic peaks were selected and proved to be effective in controlling the quality of Z. bungeanum peels with the total contribution of 92.775%. The similarity values of each sample ranged between 0.696 and 0.970. In addition, hyperoside was identified as another key chemical indicator for quality evaluation of Z. bungeanum peels by PCA. Accordingly, seven varieties of Z. bungeanum were classified into four groups, among which group 4 (Fengxian Dahongpao, S2) was considered as an outstanding variety, followed by group 1 (S5, S6, and S7) and group 3 (S3 and S4), and group 2 (Fugu late-maturing, S1) was the last one. These results will be helpful to establish an effective and comprehensive evaluation system of Z. bungeanum.Practical Application This study not only provides another index for quality evaluation of Z. bungeanum peels, but it also expects to be a theoretical basis for high-quality germplasm cultivation and the development of Z. bungeanum peels as food additives, pharmaceutical, or health care products.