Input-specific maturation of NMDAR-mediated transmission onto parvalbumin-expressing interneurons in layers 2/3 of the visual cortex
Input-specific maturation of NMDAR-mediated transmission onto parvalbumin-expressing interneurons in layers 2/3 of the visual cortex
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DOI：
10.1152/jn.00495.2018
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发表时间：
2018-12-01
影响因子：
2.5
通讯作者：
Wollmuth, Lonnie P.
中科院分区：
文献类型：
--
作者：
Ferrer, Camilo;Hsieh, Helen;Wollmuth, Lonnie P.
表达小清蛋白（PV）的GABA能中间神经元调节局部回路动力学。在驱动PV中间神经元活动的兴奋方面，PV中间神经元上的N-甲基-D-天冬氨酸受体（NMDAR）介导的组分往往小于锥体神经元上的组分，但对它们的生理和发育做出显著贡献。在视皮层，PV中间神经元在关键期成熟。我们推测，在关键时期，NMDAR介导的信号和功能特性的突触PV interneurons的发育调节。因此，我们比较了α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体（AMPAR）和NMDAR介导的突触反应之前（出生后15-20天，P15-P20），期间（P25-P40），和之后（P50-P60）的视觉关键期。AMPAR微型兴奋性突触后电流（mEPSC）的频率显示了发展下降，而NMDAR mEPSC不存在或显示极低的频率在整个发展。为了诱发反应。我们一致地看到了NMDAR介导的成分，表明诱发和自发神经传递之间的突触前或突触后差异。诱发反应表现出输入特异性的发育变化。对于intralaminar输入，NMDAR介导的组件显着减少与发展。这导致成人板内输入几乎完全由AMPAR介导，适合于精确计时的突触输入计算。并且可能具有不依赖于NMDAR的可塑性形式。与此相反，层间输入保持稳定的NMDAR介导的组件在整个发展，但有一个转变的AMPAR配对脉冲比从抑郁症的促进。促进AMPAR反应和大量NMDAR成分的成人层间输入将有利于突触反应的时间整合，并可通过NMDAR依赖形式的plasticity.NEW &值得注意的是，我们首次显示了N-甲基-D-天冬氨酸受体成分的输入特异性发育变化和兴奋性驱动对层2/3小清蛋白表达（PV）的短期可塑性。在关键时期的视觉皮层中间神经元。这些发育变化将导致功能上不同的成人板内和层间神经元能输入，这将以不同的方式参与PV中间神经元介导的抑制。
Parvalbumin-expressing (PV) GABAergic interneurons regulate local circuit dynamics. In terms of the excitation driving PV interneuron activity, the N-methyl-D-aspartate receptor (NMDAR)-mediated component onto PV interneurons tends to be smaller than that onto pyramidal neurons but makes a significant contribution to their physiology and development. In the visual cortex, PV interneurons mature during the critical period. We hypothesize that during the critical period, the NMDAR-mediated signaling and functional properties of glutamatergic synapses onto PV interneurons are developmentally regulated. We therefore compared the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-and NMDAR-mediated synaptic responses before (postnatal days 15-20, P15-P20), during (P25-P40), and after (P50-P60) the visual critical period. AMPAR miniature excitatory postsynaptic currents (mEPSCs) showed a developmental decrease in frequency, whereas NMDAR mEPSCs were absent or showed extremely low frequencies throughout development. For evoked responses. we consistently saw a NMDAR-mediated component, suggesting pre- or postsynaptic differences between evoked and spontaneous neurotransmission. Evoked responses showed input-specific developmental changes. For intralaminar inputs, the NMDAR-mediated component significantly decreased with development. This resulted in adult intralaminar inputs almost exclusively mediated by AMPARs, suited for the computation of synaptic inputs with precise timing. and likely having NMDAR-independent forms of plasticity. In contrast, interlaminar inputs maintained a stable NMDAR-mediated component throughout development but had a shift in the AMPAR paired-pulse ratio from depression to facilitation. Adult interlaminar inputs with facilitating AMPAR responses and a substantial NMDAR component would favor temporal integration of synaptic responses and could be modulated by NMDAR-dependent forms of plasticity.NEW & NOTEWORTHY We show for the first time input-specific developmental changes in the N-methyl-D-aspartate receptor component and short-term plasticity of the excitatory drive onto layers 2/3 parvalbumin-expressing (PV) interneurons in the visual cortex during the critical period. These developmental changes would lead to functionally distinct adult intralaminar and interlaminar glutamatergic inputs that would engage PV intemeuron-mediated inhibition differently.